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Updated: Nov 29, 2025
Chromosomal Spread Preparation of Human Embryonic Stem Cells for Karyotyping
Published on: September 4, 2009
G-Banded Karyotyping of Human Pluripotent Stem Cell Cultures
Erik McIntire1, Kimberly Leonhard2, Seth Taapken2
1University of Chicago, Chicago, IL, USA. emcintire@uchicago.edu.
Abstract:
Acquired chromosomal abnormalities may occur during the reprogramming and culture of human pluripotent stem cells (hPSCs). Therefore, it is required that regular testing of genetic integrity be conducted. G-banded karyotyping is a widely used genetic assay that is capable of detecting chromosomal abnormalities. Karyotyping of hPSC cultures can be a challenging undertaking for inexperienced investigators; here, we provide detailed procedures for karyotyping, including sample preparation and analysis, as well as the interpretation of hPSC karyotype results.
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