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Long-term High-Resolution Intravital Microscopy in the Lung with a Vacuum Stabilized Imaging Window
Published on: October 6, 2016
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Live Imaging of the Lung
Tomasz Brzoska1,2, Tomasz W Kaminski1, Margaret F Bennewitz3
1Pittsburgh Heart, Lung and Blood Vascular Medicine Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
Current Protocols in Cytometry
|November 23, 2020
Summary
This study updates live imaging protocols for lung tissue, improving visualization of cellular dynamics and interactions within the lung. New methods enhance quantitative analysis for studying lung biology and disease.
Area of Science:
- Pulmonary biology
- Cellular dynamics
- In vivo imaging
Background:
- Live imaging is crucial for understanding cell dynamics and interactions within tissues.
- Lung imaging is challenging due to physiological motion from breathing and heartbeat.
- Previous protocols existed for ex vivo lung slices and intact mouse lungs.
Purpose of the Study:
- To update and enhance existing protocols for live imaging of lung tissue.
- To introduce new methodologies and quantitative image analysis techniques.
- To expand potential applications for studying lung physiology and pathology.
Main Methods:
- Live imaging of ex vivo lung slices.
- Live imaging of intact mouse lungs using intratracheal and intravascular instillations.
- Quantitative analysis of neutrophil-platelet aggregation, thrombosis, and vascular permeability.
Main Results:
- Updated protocols facilitate improved live imaging of lung slices and intact mouse lungs.
- New quantitative methods allow for precise measurement of cellular interactions and vascular events.
- Demonstrated applications in analyzing pulmonary vascular dynamics and cellular aggregation.
Conclusions:
- The updated protocols provide advanced tools for high-resolution live imaging of the lung.
- These methods enable detailed investigation of cellular dynamics and pathological processes in pulmonary research.
- The enhanced techniques support quantitative analysis crucial for understanding lung health and disease.
