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Updated: Nov 29, 2025

Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis
Published on: August 12, 2009
Nuclear transformation of Chlamydomonas reinhardtii: A review
Meng-Ping Zhang1, Mou Wang1, Chuan Wang1
1College of Biotechnology, Sichuan University of Science and Engineering, Zigong, 643000, Sichuan province, China.
Abstract:
Chlamydomonas reinhardtii is a model organism with three sequenced genomes capable of genetic transformation. C. reinhardtii has the advantages of being low cost, non-toxic, and having a post-translational modification system that ensures the recombinant proteins have the same activity as natural proteins, thus making it a great platform for application in molecular biology and other fields. In this review, we summarize the existing methods for nuclear transformation of C. reinhardtii, genes for selection, examples of foreign protein expression, and factors affecting transformation efficiency, to provide insights into effective strategies for the nuclear transformation of C. reinhardtii.
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