One-pot method based on deep eutectic solvent for extraction and conversion of polydatin to resveratrol from...

Area of Science:

• Natural Product Chemistry
• Green Chemistry
• Phytochemistry

Background:

• Polygonum cuspidatum is a source of resveratrol, a valuable compound.
• Current extraction methods for resveratrol are complex, costly, and rely on organic solvents.
• Polydatin, a glycoside precursor, requires hydrolysis to yield resveratrol, adding complexity.

Purpose of the Study:

• To develop a novel, efficient, and green one-pot method for simultaneous extraction and conversion of polydatin to resveratrol.
• To optimize the extraction conditions using statistical methods.
• To evaluate the effectiveness of the developed method across different plant origins.

Main Methods:

• Development of a deep eutectic solvent (DES)-based one-pot extraction and conversion process.
• Optimization of the method using One-Variable-at-a-Time (OVAT) and response surface methodology (RSM).
• Analysis of resveratrol yield and polydatin-to-resveratrol conversion efficiency.
• Scanning electron microscopy (SEM) to investigate the mechanism of DES action on plant cell walls.

Main Results:

• The DES-based one-pot method achieved significantly higher resveratrol extraction yields compared to water, methanol, and ethanol.
• Optimized conditions yielded 12.26 ± 0.14 mg/g of resveratrol within 80 minutes.
• Conversion efficiency of polydatin to resveratrol exceeded 96.3% across samples from five different origins.
• SEM confirmed that the DES disrupted plant cell walls, enhancing resveratrol release.

Conclusions:

• A green and efficient one-pot method using DES was successfully established for resveratrol production from Polygonum cuspidatum.
• This novel approach simplifies the extraction process and improves yield, offering a sustainable alternative to conventional methods.
• The method demonstrates high conversion efficiency and broad applicability across different plant sources.