Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

SNAREs and Membrane Fusion01:43

SNAREs and Membrane Fusion

11.9K
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
11.9K
Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

4.4K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
4.4K
Bacterial Phylum Chlamydiae01:29

Bacterial Phylum Chlamydiae

275
The phylum Chlamydiae or Chlamydiota is composed of a single order, Chlamydiales. This phylum consists entirely of obligate intracellular parasites that infect eukaryotic hosts. While human pathogens within this group have been studied extensively, the phylum encompasses many species capable of interacting with various eukaryotic organisms. Members of Chlamydiae are typically small cocci, approximately 0.5 μm in diameter, and exhibit a distinctive developmental cycle. As is characteristic...
275
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

8.5K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
8.5K
The Replisome03:01

The Replisome

37.3K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
37.3K
Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

2.8K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TRP120-dependent activation of noncanonical Wnt/NFAT signaling drives monocyte chemokine production in <i>Ehrlichia chaffeensis</i> infection.

mSphere·2026
Same author

CRISPRi and beyond: studying essential gene function in the obligate intracellular bacterium <i>Chlamydia trachomatis</i>.

Journal of bacteriology·2026
Same author

Coordinated topoisomerase function shapes the fluoroquinolone response of <i>Chlamydia trachomatis</i>.

bioRxiv : the preprint server for biology·2026
Same author

Characterization of the cell division-associated peptidoglycan amidase AmiA of <i>Chlamydia trachomatis</i>.

Journal of bacteriology·2026
Same author

Characterization of the Cell Division-Associated Peptidoglycan Amidase AmiA of <i>Chlamydia trachomatis</i>.

bioRxiv : the preprint server for biology·2025
Same author

TRP75-mediated STAT3 activation promotes anti-apoptotic signaling and <i>Ehrlichia chaffeensis</i> infection.

Infection and immunity·2025

Related Experiment Video

Updated: Nov 29, 2025

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
09:19

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

Published on: October 19, 2012

14.3K

Eukaryotic SNARE VAMP3 Dynamically Interacts with Multiple Chlamydial Inclusion Membrane Proteins.

Duc-Cuong Bui1, Lisa M Jorgenson1, Scot P Ouellette1

  • 1Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA.

Infection and Immunity
|November 24, 2020
PubMed
Summary

Chlamydia trachomatis uses type III secreted proteins (Incs) to interact with host VAMP3 and VAMP4 proteins, facilitating its developmental cycle. This study identifies transient Inc-VAMP interactions crucial for chlamydial pathogenesis.

Keywords:
Chlamydia trachomatisSNAREhost-pathogen interactionsinclusion membrane proteinobligate intracellular pathogen

More Related Videos

Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells
07:42

Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells

Published on: October 13, 2015

7.8K
Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
08:55

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Published on: December 29, 2017

9.9K

Related Experiment Videos

Last Updated: Nov 29, 2025

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
09:19

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

Published on: October 19, 2012

14.3K
Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells
07:42

Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells

Published on: October 13, 2015

7.8K
Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
08:55

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Published on: December 29, 2017

9.9K

Area of Science:

  • Microbiology
  • Cell Biology
  • Pathogen-Host Interactions

Background:

  • Chlamydia trachomatis is an obligate intracellular pathogen with a biphasic developmental cycle.
  • Chlamydial inclusions are modified by type III secreted proteins (Incs) for host cell interaction.
  • Eukaryotic SNARE proteins VAMP3 and VAMP4 are recruited to the inclusion for expansion, requiring de novo chlamydial protein synthesis.

Purpose of the Study:

  • To identify specific Inc binding partners for VAMP3 and VAMP4.
  • To investigate the hypothesis that Incs recruit VAMP3 and VAMP4.
  • To characterize the transient nature of chlamydia-host protein interactions.

Main Methods:

  • Utilized two complementary experimental systems to identify Inc-VAMP binding partners.
  • Created transformed Chlamydia trachomatis strains for inducible Inc-FLAG protein expression.
  • Analyzed VAMP3 localization in infected cells with altered Inc expression.

Main Results:

  • Identified five Incs that transiently interact with VAMP3 during infection.
  • Demonstrated that loss of incA or ct813 expression affects VAMP3 localization to the inclusion.
  • Revealed the transient nature of specific host protein-Inc interactions.

Conclusions:

  • Certain Incs transiently interact with VAMP3 to facilitate the chlamydial developmental cycle.
  • IncA and CT813 play roles in VAMP3 recruitment to the chlamydial inclusion.
  • This study highlights the dynamic nature of host-pathogen interactions in chlamydial infections.