Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

COXFA4L2 upregulation preserves residual cytochrome c oxidase activity in COXFA4-related Leigh-like encephalopathy.

Nature communications·2026
Same author

The cytochrome c oxidase subunit COX6B1 is required for redox-sensitive early assembly and late stabilization of complex IV.

The Journal of biological chemistry·2025
Same author

The homoplasmic MT-TK m.8357T > C mtDNA variant as a cause of multiorgan mitochondrial disease.

Mitochondrion·2025
Same author

Platelet mitochondrial complex I and IV activities are not reliable stratification biomarkers in Parkinson's disease.

Journal of Parkinson's disease·2025
Same author

Uncovering a Novel Pathogenic Mechanism of <i>BCS1L</i> in Mitochondrial Disorders: Insights from Functional Studies on the c.38A>G Variant.

International journal of molecular sciences·2025
Same author

Mitochondrial complex I deficiency occurs in skeletal muscle of a subgroup of individuals with Parkinson's disease.

Communications medicine·2025

Related Experiment Video

Updated: Nov 29, 2025

Analysis of Mitochondrial Respiratory Chain Complexes in Cultured Human Cells using Blue Native Polyacrylamide Gel Electrophoresis and Immunoblotting
07:55

Analysis of Mitochondrial Respiratory Chain Complexes in Cultured Human Cells using Blue Native Polyacrylamide Gel Electrophoresis and Immunoblotting

Published on: February 12, 2019

16.4K

Blue-Native Electrophoresis to Study the OXPHOS Complexes.

Erika Fernandez-Vizarra1, Massimo Zeviani2

  • 1MRC Mitochondrial Biology Unit, University of Cambridge, Cambridge, UK. emfvb2@mrc-mbu.cam.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|November 24, 2020
PubMed
Summary

Blue-native polyacrylamide gel electrophoresis (BN-PAGE) analyzes mitochondrial oxidative phosphorylation (OXPHOS) complexes by preserving subunit interactions. This method is crucial for understanding OXPHOS disorders in various organisms.

Keywords:
Blue-native gel electrophoresisFirst-dimension BN-PAGEIn gel activity assaysMitochondrial complexes I, II, III, IV, and VOxidative phosphorylation systemSecond-dimension BN-PAGE

More Related Videos

Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes
11:25

Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes

Published on: May 19, 2019

14.1K
Analyzing Supercomplexes of the Mitochondrial Electron Transport Chain with Native Electrophoresis, In-gel Assays, and Electroelution
08:37

Analyzing Supercomplexes of the Mitochondrial Electron Transport Chain with Native Electrophoresis, In-gel Assays, and Electroelution

Published on: June 1, 2017

14.5K

Related Experiment Videos

Last Updated: Nov 29, 2025

Analysis of Mitochondrial Respiratory Chain Complexes in Cultured Human Cells using Blue Native Polyacrylamide Gel Electrophoresis and Immunoblotting
07:55

Analysis of Mitochondrial Respiratory Chain Complexes in Cultured Human Cells using Blue Native Polyacrylamide Gel Electrophoresis and Immunoblotting

Published on: February 12, 2019

16.4K
Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes
11:25

Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes

Published on: May 19, 2019

14.1K
Analyzing Supercomplexes of the Mitochondrial Electron Transport Chain with Native Electrophoresis, In-gel Assays, and Electroelution
08:37

Analyzing Supercomplexes of the Mitochondrial Electron Transport Chain with Native Electrophoresis, In-gel Assays, and Electroelution

Published on: June 1, 2017

14.5K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Mitochondrial oxidative phosphorylation (OXPHOS) is vital for cellular energy production.
  • Analyzing intact OXPHOS complexes is challenging due to their sensitivity to disruption.
  • Understanding OXPHOS function and dysfunction is key in various pathologies.

Purpose of the Study:

  • To highlight the utility of Blue-native polyacrylamide gel electrophoresis (BN-PAGE) for OXPHOS analysis.
  • To explain the principles behind BN-PAGE in preserving protein complex integrity.
  • To underscore the significance of BN-PAGE in studying mitochondrial diseases.

Main Methods:

  • Blue-native polyacrylamide gel electrophoresis (BN-PAGE) is employed.
  • Mild detergents are used to extract mitochondrial inner membrane complexes.
  • Electrophoresis is performed without denaturing agents to maintain complex structure.
  • Downstream detection techniques are combined with BN-PAGE.

Main Results:

  • BN-PAGE effectively separates the five main OXPHOS complexes.
  • The technique preserves interactions between subunits within integral complexes.
  • Extensive data has been generated using BN-PAGE since the 1990s.
  • BN-PAGE has been applied across diverse organisms for OXPHOS studies.

Conclusions:

  • BN-PAGE is an optimized technique for analyzing mitochondrial OXPHOS.
  • The method's ability to preserve complex integrity is critical.
  • BN-PAGE is instrumental in advancing the understanding of OXPHOS physiopathology.