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Formononetin exhibits anticancer activity in gastric carcinoma cell and regulating miR-542-5p

Wei-Song Wang1, Can-Song Zhao2

  • 1Department of General Surgery, Zhuji Central Hospital, Zhuji, China.

The Kaohsiung Journal of Medical Sciences
|November 24, 2020
PubMed

Insights

Formononetin effectively inhibits gastric carcinoma (GC) growth and spread by reducing cell viability and migration. This natural compound also downregulates the oncogene microRNA-542-5p (miR-542-5p), offering a potential therapeutic strategy for GC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Formononetin is known for anti-neoplastic effects in colon and breast cancers.
  • The role of formononetin in gastric carcinoma (GC) remains largely unexplored.
  • Gastric carcinoma exhibits aggressive growth and metastatic potential.

Purpose of the Study:

  • To investigate the anticancer effects of formononetin on gastric carcinoma cells.
  • To elucidate the underlying molecular mechanisms, including the role of microRNA-542-5p (miR-542-5p).
  • To evaluate formononetin's efficacy in preclinical models of GC.

Main Methods:

  • Cell viability assays on GC cell lines (SGC-7901, MGC-803).
  • Migration and invasion assays for GC cells treated with formononetin.
  • In vivo studies using a xenograft model to assess anticancer effects.
  • Analysis of microRNA-542-5p (miR-542-5p) expression in GC.

Main Results:

  • Formononetin significantly reduced the viability of SGC-7901 and MGC-803 GC cell lines.
  • Formononetin suppressed the migration and invasion capabilities of GC cells in vitro.
  • Anticancer effects were confirmed in a xenograft model.
  • Upregulation of microRNA-542-5p (miR-542-5p) was observed in GC, and it may mediate formononetin's activity.

Conclusions:

  • Formononetin demonstrates potent inhibitory effects on gastric carcinoma cell growth and aggressiveness.
  • The compound's action involves the modulation of microRNA-542-5p (miR-542-5p).
  • Formononetin presents a promising therapeutic candidate for gastric carcinoma treatment.
Keywords:
formononetingastric carcinomainvasionmiR-542-5pmigration

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