Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Development of a Theoretical Framework for Program Evaluation of the Impact of Policy, Systems, and Environmental Interventions on Individual-Level Behavior.

Journal of human nutrition and dietetics : the official journal of the British Dietetic Association·2026

Same author

Synergistic immunomodulatory combinations induce robust human tolerogenic dendritic cells for antigen-specific regulatory T cell generation.

The Journal of pharmacology and experimental therapeutics·2026

Same author

Vital Sign and Nursing Assessment Patterns in Emergency Transfers to the Intensive Care Unit.

Hospital pediatrics·2026

Same author

Rewiring Oncogenic Transcriptional Complexes with Domain-ALTeration Chimeras (DALTACs) in Prostate Cancer.

bioRxiv : the preprint server for biology·2026

Same author

STAG2 loss amplifies EWS-FLI1-driven microsatellite enhancer activity promoting Ewing sarcoma aggressiveness.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

ZFTA-RELA ependymomas make itaconate to epigenetically drive fusion expression.

Nature·2026

Search research articles

Related Experiment Video

Updated: May 1, 2026

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

Customized optical mapping by CRISPR-Cas9 mediated DNA labeling with multiple sgRNAs.

Heba Z Abid¹, Eleanor Young¹, Jennifer McCaffrey¹

¹School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA, USA.

Nucleic Acids Research

|November 24, 2020

Summary

CRISPR-Cas9 genome editing enables novel DNA labeling for precise genome mapping. This technique differentiates single-nucleotide variations and defines structural variants in complex genomes.

More Related Videos

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Published on: May 28, 2019

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

Related Experiment Videos

Last Updated: May 1, 2026

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Published on: May 28, 2019

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

Area of Science:

Genomics
Molecular Biology
Biotechnology

Background:

Whole-genome mapping is crucial for genome assembly and structural variation analysis.
CRISPR-Cas9 genome editing offers a novel DNA labeling strategy targeting specific sequences.
Existing methods have limitations in labeling repetitive or inaccessible DNA regions.

Purpose of the Study:

To develop customized CRISPR-Cas9 DNA labeling strategies for advanced genome mapping.
To enhance the ability to interrogate single-allele differences and conserved sequences.
To create genome-wide mapping patterns using multiple single-guide RNAs (sgRNAs).

Main Methods:

Designed a CRISPR-Cas9 strategy to differentiate single allele differences at NGG protospacer adjacent motifs (PAM).
Combined CRISPR-Cas9 labeling with sequence motif analysis to identify single-base differences.
Developed a method for single-tube synthesis of multiple sgRNAs for multiplexed labeling.
Applied 162 sgRNAs to map the 2Mb Haemophilus influenzae chromosome.

Main Results:

Demonstrated the ability to pinpoint single-base differences in conserved sequences.
Successfully mapped the Haemophilus influenzae chromosome using a multiplexed CRISPR-Cas9 labeling approach.
Showcased the utility of CRISPR-Cas9 mapping for interrogating specific genomic loci.

Conclusions:

CRISPR-Cas9 DNA labeling provides a versatile tool for high-resolution genome mapping.
These methods are valuable for defining long-distance haplotypes and structural variant breakpoints.
The approach is applicable to complex genomes and microbial mixtures, advancing genomic analysis.