Glucose, insulin, and cortisol concentrations and glucose tolerance test in Holstein cows with inactive ovaries

A total of 60 healthy (the control group) and 30 anestrous Holstein cows with inactive ovaries (the case group) from three dairy farms around Tehran were randomly selected and diagnosed to determine the concentrations of glucose, insulin, and cortisol, and the rate of the insulin resistance disorder in them. Primarily, serum samples were obtained from all animals, and the concentrations of glucose, insulin, and cortisol were measured in a laboratory. Thereupon, the glucose tolerance test (GTT) was implemented. Before the implementation of the GTT, the median of serum glucose levels (62 mg/dl), insulin (7.16 μIU/ml), and cortisol (2.5 μg/dl) was estimated in healthy cows. The median of serum glucose levels (68.5 mg/dl, p = 0.00009) was higher, and serum insulin (6.1 μIU/ml, p = 0.025) and cortisol (1.1 μg/dl, p = 0.00029) levels were lesser in the anestrous cows when estimated simultaneously. After the implementation of GTT, the median of serum glucose concentrations in one (120 mg/dl), two (73.5 mg/dl), and three (63.5 mg/dl) hours after the glucose injection was estimated in healthy cows. The findings also showed that the median of serum glucose levels was lesser in one (100 mg/dl, p = 0.015), similar in two (77.5 mg/dl, p = 0.9), and higher in three (70 mg/dl, p = 0.012) hours after the glucose injection in anestrous cows. In conclusion, higher serum glucose, lesser insulin and cortisol concentrations, and the different correlation between serum glucose and insulin levels before the GTT were detected in anestrous cows. The pattern of glucose changes also differed in anestrous cows after the GTT.