Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

14.1K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
14.1K
Lineage Commitment01:21

Lineage Commitment

3.7K
Commitment is the  process whereby stem cells:
3.7K
Combinatorial Gene Control02:33

Combinatorial Gene Control

9.0K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.0K
General Transcription Factors01:30

General Transcription Factors

6.4K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
6.4K
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

7.5K
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
7.5K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

8.2K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
8.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pathogenesis of diffuse large B cell lymphoma proteogenotypes.

Cancer cell·2026
Same author

SPEN loss drives extra-follicular diffuse large B cell lymphoma with female-specific lethality and therapeutic vulnerabilities.

Cancer discovery·2026
Same author

Enhanced efficacy of a specific HDAC3 inhibitor in combination with 5-azacitidine against diffuse large B-cell lymphoma.

Blood neoplasia·2026
Same author

Elevated levels of TNF and its targets characterize better-risk older acute myeloid leukemia patients.

Leukemia·2026
Same author

CCAAT-enhancer binding protein delta functions as a tumor suppressor gene in acute myeloid leukemia.

Neoplasia (New York, N.Y.)·2026
Same author

Anti-programmed cell death protein 1-based salvage therapy for relapsed/refractory Hodgkin lymphoma: a multicenter real-world analysis.

Haematologica·2026

Related Experiment Video

Updated: Nov 29, 2025

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
13:55

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Published on: February 3, 2013

18.8K

Unique Immune Cell Coactivators Specify Locus Control Region Function and Cell Stage.

Chi-Shuen Chu1, Johannes C Hellmuth2, Rajat Singh2

  • 1Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY 10065, USA.

Molecular Cell
|November 24, 2020
PubMed
Summary
This summary is machine-generated.

OCA-B forms a complex with OCT2 and MEF2B to activate a locus control region (LCR) essential for B cell identity and function. This complex is crucial for regulating the BCL6 proto-oncogene in normal and malignant germinal center B cells.

Keywords:
B cell lymphomaBCL6CRISPRMEF2BMediator complexOCA-BOCT2enhancergerminal centerlocus control region

More Related Videos

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.5K
Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR
08:02

Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR

Published on: February 19, 2020

7.4K

Related Experiment Videos

Last Updated: Nov 29, 2025

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
13:55

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Published on: February 3, 2013

18.8K
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.5K
Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR
08:02

Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR

Published on: February 19, 2020

7.4K

Area of Science:

  • Molecular Biology
  • Immunology
  • Genetics

Background:

  • Locus control regions (LCRs) are critical for cellular identity, but their structural hierarchy and functional drivers are not fully understood.
  • Understanding LCR function is vital for insights into diseases like cancer.

Purpose of the Study:

  • To elucidate the molecular mechanisms underlying LCR function in B cells.
  • To identify the specific factors and regulatory elements involved in activating B cell-specific LCRs.

Main Methods:

  • Formation and characterization of a ternary complex involving OCA-B, OCT2, and MEF2B.
  • Analysis of LCR occupancy and activation of the BCL6 proto-oncogene.
  • CRISPR interference (CRISPRi) screening to identify essential LCR segments.

Main Results:

  • OCA-B forms a ternary complex with OCT2 and MEF2B, which binds and activates an LCR regulating the BCL6 proto-oncogene.
  • This complex is essential for normal and malignant germinal center (GC) B cell function.
  • The complex facilitates Mediator recruitment to the BCL6 promoter via OCA-B-MED1 interactions.
  • CRISPRi screening confirmed that only LCR segments with high ternary complex binding are functionally essential.

Conclusions:

  • A complex of lineage- and stage-specific factors (OCA-B, OCT2, MEF2B) converges on key enhancer elements to drive cell-type-defining LCR function.
  • This mechanism highlights the intricate regulation of B cell identity and the BCL6 proto-oncogene.