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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

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Implementation of High-Throughput Sequencing (HTS) in Aptamer Selection Technology.

Natalia Komarova¹, Daria Barkova¹, Alexander Kuznetsov¹

¹Scientific-Manufacturing Complex Technological Centre, 1-7 Shokin Square, Zelenograd, 124498 Moscow, Russia.

International Journal of Molecular Sciences

|November 25, 2020

Summary

Systematic evolution of ligands by exponential enrichment (SELEX) uses high-throughput sequencing (HTS) for aptamer discovery. This integration enhances aptamer identification and analysis for diverse applications.

Keywords:

SELEX aptamer evolution high-throughput sequencing next-generation sequencing random library

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Area of Science:

Biotechnology
Molecular Biology
Genomics

Background:

Aptamers are nucleic acid ligands with specific binding capabilities.
Their potential in diagnostics, therapeutics, and analysis is rapidly growing.
Systematic evolution of ligands by exponential enrichment (SELEX) is a key method for aptamer isolation.

Purpose of the Study:

To discuss the technical aspects of integrating high-throughput sequencing (HTS) with SELEX.
To explore the potential of HTS-SELEX for aptamer identification and characterization.
To highlight the benefits of HTS in analyzing large aptamer libraries.

Main Methods:

The review focuses on the SELEX procedure for aptamer isolation.
High-throughput sequencing (HTS) is presented as a method to analyze large nucleic acid libraries.
Data analysis from HTS of SELEX-generated libraries is discussed.

Main Results:

HTS effectively addresses the extensive sequencing demands of SELEX.
Analysis of HTS data aids in precise aptamer identification.
HTS provides insights into the structure and function of nucleic acid ligands.

Conclusions:

Integrating HTS with SELEX significantly enhances aptamer discovery and characterization.
This combined approach is crucial for advancing aptamer-based applications.
HTS-SELEX offers a powerful platform for understanding nucleic acid-target interactions.