Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Drugs that Stabilize Microtubules01:15

Drugs that Stabilize Microtubules

2.4K
Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...
2.4K
Factors Affecting Dissolution: Polymorphism, Amorphism and Pseudopolymorphism01:21

Factors Affecting Dissolution: Polymorphism, Amorphism and Pseudopolymorphism

528
Polymorphism refers to the existence of a drug substance in multiple crystalline forms, known as polymorphs. Recently, this term has been expanded to include solvates (forms containing a solvent), amorphous forms (non-crystalline forms), and desolvated solvates (forms from which the solvent has been removed).
Some polymorphic crystals possess lower aqueous solubility than their amorphous counterparts, leading to incomplete absorption. For instance, the oral suspension of Chloramphenicol, which...
528
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.7K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.7K
Pharmaceutical Alternatives: Polymorphic Form-Related and Particle Size-Related Therapeutic Nonequivalence01:27

Pharmaceutical Alternatives: Polymorphic Form-Related and Particle Size-Related Therapeutic Nonequivalence

80
Changes in polymorphic forms can significantly influence the bioavailability of poorly soluble drugs. Although the FDA defines pharmaceutical equivalence based on having the same active ingredient, dosage form, and route of administration, it does not automatically disqualify products with different polymorphic forms. This means two products with different polymorphs can still be deemed pharmaceutically equivalent. However, polymorphic differences can affect properties like wettability,...
80
Protein Complex Assembly02:41

Protein Complex Assembly

15.8K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
15.8K
Globular and Fibrous Proteins02:21

Globular and Fibrous Proteins

46.2K
Many proteins can be classified into two distinct subtypes - globular or fibrous. These two types differ in their shapes and solubilities.
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
46.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthesis and Perspectives of Oriented Growth of Double-Perovskite Cs<sub>2</sub>SnI<sub>6</sub> in the Presence of Antimony.

Nanomaterials (Basel, Switzerland)·2026
Same author

Dihydroquercetin in Obesity and Prediabetes: Case Report and Insights from Molecular Modeling.

International journal of molecular sciences·2026
Same author

Co-Amorphous Systems Based on Dihydroquercetin and l-Lysine: Synthesis and Evaluation.

Pharmaceutics·2025
Same author

Dihydroquercetin in Weight Control: Systematic Review and Meta-Analysis of Preclinical Studies.

Pharmaceuticals (Basel, Switzerland)·2025
Same author

Modification of biopharmaceutical parameters of flavonoids: a review.

Frontiers in chemistry·2025
Same author

Insights in wound healing properties of water-soluble composition of dihydroquercetin and L-lysine.

Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques·2025

Related Experiment Video

Updated: Nov 28, 2025

Author Spotlight: Genetic Profiling for Fluorouracil Response in Gastric Cancer
06:21

Author Spotlight: Genetic Profiling for Fluorouracil Response in Gastric Cancer

Published on: May 10, 2024

997

Assembling the Puzzle of Taxifolin Polymorphism.

Roman P Terekhov1, Irina A Selivanova1, Nonna A Tyukavkina1

  • 1Department of Chemistry, Sechenov First Moscow State Medical University, Trubetskaya st. 8-2, 119991 Moscow, Russia.

Molecules (Basel, Switzerland)
|November 25, 2020
PubMed
Summary

Polymorphism in flavonoids like taxifolin is crucial for active pharmaceutical ingredients (APIs). This study confirms taxifolin microtubes are a pseudopolymorphic form, clarifying its solid-state behavior.

Keywords:
X-ray diffractionactive pharmaceutical ingredientflavonoidspolymorphismscanning electron microscopytaxifolinthermal analysisvariable-temperature powder X-ray diffraction

More Related Videos

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene
07:00

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

Published on: April 1, 2019

10.2K
Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

5.5K

Related Experiment Videos

Last Updated: Nov 28, 2025

Author Spotlight: Genetic Profiling for Fluorouracil Response in Gastric Cancer
06:21

Author Spotlight: Genetic Profiling for Fluorouracil Response in Gastric Cancer

Published on: May 10, 2024

997
A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene
07:00

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

Published on: April 1, 2019

10.2K
Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

5.5K

Area of Science:

  • Pharmaceutical Science
  • Solid-State Chemistry
  • Materials Science

Background:

  • Polymorphism is a critical aspect of active pharmaceutical ingredients (APIs) solid-state characterization.
  • Flavonoids, including taxifolin (dihydroquercetin), are extensively studied for their polymorphic potential.
  • Previous reports on new taxifolin forms lacked definitive polymorphic classification.

Purpose of the Study:

  • To investigate and confirm the polymorphism of taxifolin.
  • To characterize novel taxifolin microstructures (microtubes and microspheres) synthesized via crystal engineering.
  • To elucidate the solid-state nature of these taxifolin forms.

Main Methods:

  • Synthesis of taxifolin microtubes and microspheres using crystal engineering techniques.
  • Characterization using scanning electron microscopy (SEM) for morphology.
  • Analysis via X-ray powder diffraction (XRPD) for crystalline structure.
  • Thermal analysis (TA) to assess thermal behavior.
  • Spectroscopic methods for chemical identification.

Main Results:

  • SEM revealed distinct morphologies for raw taxifolin, microtubes, and microspheres.
  • XRPD patterns of raw taxifolin and microtubes were similar, but thermal analysis (TA) profiles differed significantly.
  • Raw taxifolin and microspheres exhibited similar thermograms, yet XRPD indicated crystalline (raw) and amorphous (microspheres) states.
  • Combined analytical techniques were essential to confirm taxifolin polymorphism.

Conclusions:

  • Taxifolin exhibits polymorphism, a critical factor in its API solid-state properties.
  • Taxifolin microtubes represent a pseudopolymorphic modification of the raw crystalline form.
  • The study highlights the necessity of employing a combination of analytical methods for accurate solid-state characterization of APIs.