Related Experiment Video

Updated: Aug 5, 2026

Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery
10:03

Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery

Published on: November 5, 2019

Endogenous production of infectious Inoue-Melnick virus in a human meningioma cell line

Y Nishibe1, Y K Inoue, A C Hollinshead

  • 1Institute for Virus Research, Kyoto University, Japan.

Journal of Medical Virology
|November 1, 1987
PubMed

Insights

Inoue-Melnick virus (IMV) production was studied in human meningioma-derived MG-1 cells. Results show continuous IMV replication and extracellular release, with over 90% of cells expressing viral antigens.

Area of Science:

  • Virology
  • Cell Biology
  • Oncology

Background:

  • The MG-1 cell line originates from human meningioma tissue.
  • Inoue-Melnick virus (IMV) is a type 1 virus with implications for cell culture studies.

Purpose of the Study:

  • To investigate the continuous production of Inoue-Melnick virus (IMV) in the MG-1 cell line.
  • To characterize the replication and release of IMV within this specific cell line.

Main Methods:

  • Cell culture of MG-1 cells derived from human meningioma.
  • Infectious virus recovery assays.
  • Infective center assays.
  • Immunofluorescence staining for IMV-associated cytoplasmic antigens (IMCA).

Main Results:

  • Continuous production of IMV was achieved in the MG-1 cell line.
  • Infectious IMV was predominantly recovered extracellularly.
  • Over 90% of MG-1 cells exhibited IMV-associated cytoplasmic antigens (IMCA) via immunofluorescence.
  • Infective center assays confirmed widespread viral production capability among MG-1 cells.

Conclusions:

  • The MG-1 cell line supports robust and continuous replication of Inoue-Melnick virus.
  • MG-1 cells are highly susceptible to IMV infection, with most cells actively producing and releasing the virus.
  • This cell line serves as a valuable model for studying IMV biology and pathogenesis.

More Related Videos

Induction of Leptomeningeal Cells Modification Via Intracisternal Injection05:55

Induction of Leptomeningeal Cells Modification Via Intracisternal Injection

Published on: May 7, 2020

Neisseria meningitidis Infection of Induced Pluripotent Stem-Cell Derived Brain Endothelial Cells10:12

Neisseria meningitidis Infection of Induced Pluripotent Stem-Cell Derived Brain Endothelial Cells

Published on: July 14, 2020

Related Concept Videos

Viral Meningitis01:18

Viral Meningitis

Viral meningitis is the most common form of meningitis and is often referred to as aseptic meningitis to indicate the absence of bacterial involvement. It is generally milder than bacterial meningitis, with symptoms including fever, headache, stiff neck, drowsiness, nausea, photophobia, and vomiting. Rarely, more severe manifestations or death may occur. Common causative agents include enteroviruses, particularly coxsackie A and B viruses and echoviruses, all members of the Enterovirus genus...