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Development of a practicable method for isolation and identification of dengue viruses in developing countries
M Nawa1, C A Torres, F J Paladin
1Department of Microbiology, Saitama Medical School, Japan.
Japanese Journal of Medical Science & Biology
|April 1, 1987
Abstract:
We sought a practicable method for isolation and identification of dengue viruses in South-East Asia. We compared two mosquito cell lines, C6/36 and TRA-284-SFG, for virus isolation and two identification methods, immunofluorescent staining of infected cells with serotype-specific mouse monoclonal antibodies and a sandwich-type enzyme-linked immunosorbent assay with conventional mouse hyperimmune ascitic fluids. We found that the combination of TRA-284-SFG cells and ELISA is a useful and feasible method in developing countries.