Related Experiment Video

Updated: Nov 28, 2025

Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis
08:55

Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis

Published on: August 7, 2018

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Cytoplasmic MYC is an anti-necroptotic protein

Eun-Woo Lee1, Daehyeon Seong2, Jaewhan Song2

  • 1Metabolic Regulation Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.

Molecular & Cellular Oncology
|November 25, 2020
PubMed

Insights

Cancer cells resist cell death pathways like necroptosis. Our study reveals the oncogene MYC directly inhibits receptor-interacting protein kinase 3 (RIPK3), blocking necroptosis initiation.

Area of Science:

  • Molecular Biology
  • Oncology
  • Cell Death Research

Background:

  • Cancer cells frequently evade programmed cell death, including apoptosis and necroptosis.
  • The precise mechanisms underlying this resistance are not fully elucidated.
  • Receptor-interacting protein kinase 3 (RIPK3) is crucial for initiating necroptosis.

Purpose of the Study:

  • To investigate the crosstalk between the oncogene MYC and RIPK3 in the context of cancer cell death resistance.
  • To elucidate the molecular mechanisms by which MYC influences necroptosis.

Main Methods:

  • Biochemical assays to detect direct binding between MYC and RIPK3.
  • Cell-based experiments to assess the impact of MYC on necrosome formation and necroptosis induction.
  • Analysis of MYC localization within the cytoplasm.

Main Results:

  • Demonstrated direct physical interaction between cytoplasmic MYC and RIPK3.
  • Showed that MYC binding to RIPK3 inhibits the formation of the necrosome complex.
  • Confirmed that MYC actively suppresses the initiation of necroptosis in cancer cells.

Conclusions:

  • MYC directly inhibits RIPK3-mediated necroptosis by preventing necrosome assembly.
  • This interaction provides a novel mechanism for cancer cell survival and resistance to cell death.
  • Targeting the MYC-RIPK3 interaction may offer new therapeutic strategies against cancer.
Keywords:
MYCRIPK1RIPK3TNFnecroptosis

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