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High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay
Published on: January 7, 2019
On-Site Colorimetric Detection of Cholesterol Based on Polypyrrole Nanoparticles
Chengyi Hong1, Xiaoxia Zhang1, Chenyue Wu1
1College of Food and Biological Engineering, Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, Xiamen 361021, China.
Abstract:
Herein, we report a facile method for cholesterol detection by coupling the peroxidase-like activity of polypyrrole nanoparticles (PPy NPs) and cholesterol oxidase (ChOx). ChOx can catalyze the oxidation of cholesterol to produce H2O2. Subsequently, PPy NPs, as a nanozyme, induce the reaction between H2O2 and 3,3',5,5'-tetramethylbenzidine (TMB). Under optimal conditions, the increase is proportional to cholesterol with concentrations from 10 to 800 μM in absorbance of TMB at 652 nm. The linear range for cholesterol is 10-100 μM, with a detection limit of 3.5 μM. This reported method is successfully employed for detection of cholesterol in human serum. The recovery percentage is ranged within 96-106.9%. Furthermore, we designed a facile and simple portable assay kit using the proposed system, realizing the on-site semiquantitative and visual detection of cholesterol in human serum. The cholesterol content detected from the portable assay kit were closely matching those obtained results from solution-based assays, thereby holding great potential in clinical diagnosis and health management.
