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Updated: Nov 28, 2025
In vivo Dual Substrate Bioluminescent Imaging
Published on: October 11, 2011
Coelenterazine Analogue with Human Serum Albumin-Specific Bioluminescence
Ryo Nishihara1,2, Kazuki Niwa1, Tatsunosuke Tomita1,2
1National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
Abstract:
A synthetic luciferin comprising an imidazopyrazinone core, named HuLumino1, was designed to generate specific bioluminescence with human serum albumin (HSA) in real serum samples. HuLumino1 was developed by attaching a methoxy-terminated alkyl chain to C-6 of coelenterazine and by eliminating a benzyl group at C-8. HSA levels were quantified within 5% error margins of an enzyme-linked immunosorbent assay without the need for any sample pretreatments because of the high specificity of HuLumino1.
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