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Long noncoding RNA LINC00982 upregulates CTSF expression to inhibit gastric cancer progression via the transcription

Lei Zheng1, Junlin Cao1, Lijie Liu1

  • 1Department of Oncology, the First Hospital of Qinhuangdao, Qinhuangdao, China.

American Journal of Physiology. Gastrointestinal and Liver Physiology
|November 25, 2020
PubMed

Insights

Long noncoding RNA LINC00982 acts as a tumor suppressor in gastric cancer by inhibiting proliferation and metastasis. It targets HEY1, promoting cathepsin F (CTSF) expression, offering a potential therapeutic target for gastric cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Gastric cancer malignancy is influenced by long noncoding RNAs (lncRNAs).
  • The specific role and regulatory mechanisms of LINC00982 in gastric cancer remain unclear.
  • Understanding lncRNA functions is crucial for developing novel cancer therapies.

Purpose of the Study:

  • To elucidate the molecular mechanisms by which LINC00982 regulates gastric cancer cell malignancy.
  • To investigate the interaction between LINC00982, HEY1, and CTSF in gastric cancer.
  • To evaluate LINC00982 as a potential therapeutic target for gastric cancer.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) and Western blotting to assess LINC00982 and CTSF expression.
  • Cellular assays including CCK-8, colony formation, Transwell migration, and invasion assays to evaluate LINC00982's functional effects.
  • RNA-binding protein immunoprecipitation, luciferase assays, and chromatin immunoprecipitation to determine molecular interactions.
  • In vivo tumor formation assays in a nude mouse model.

Main Results:

  • LINC00982 expression was downregulated in gastric cancer tissues and cells.
  • Overexpression of LINC00982 suppressed gastric cancer cell proliferation, migration, and invasion.
  • LINC00982 directly bound to transcription factor HEY1, inhibiting its expression.
  • LINC00982 promoted CTSF expression by disrupting HEY1 binding to the CTSF promoter.
  • In vivo studies confirmed the tumor-suppressive role of LINC00982.

Conclusions:

  • LINC00982 functions as a tumor suppressor in gastric cancer.
  • The regulatory network involving LINC00982, HEY1, and CTSF provides insights into gastric tumorigenesis.
  • LINC00982 represents a promising novel antitumor target for gastric cancer treatment.
Keywords:
HEY1LINC00982cathepsin Fgastric cancerlong noncoding RNAmigrationproliferation

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