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Western Blotting

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Related Experiment Video

Updated: Nov 28, 2025

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

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Published on: January 6, 2016

Global Detection of Proteins by Label-Based Antibody Array.

Zhizhou Kuang¹, Li-Pai Chen², Ruochun Huang³

¹RayBiotech Life, Peachtree Corners, GA, USA. Ken@raybiotech.com.

Methods in Molecular Biology (Clifton, N.J.)

|November 25, 2020

Summary

Label-based arrays overcome limitations of traditional antibody arrays by directly labeling all sample targets. This approach enhances proteomic detection breadth, enabling broader analysis of biological samples.

Keywords:

Antibody array Biotin labeling

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Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

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Published on: May 4, 2012

Area of Science:

Biotechnology
Proteomics
Assay Development

Background:

Traditional sandwich-based antibody arrays face limitations due to scarce antibody pairs and cross-reactivity.
These issues restrict array density and reduce the breadth of proteomic detection.
Developing high-density arrays with broad proteomic coverage remains a significant challenge.

Purpose of the Study:

To introduce label-based array technology as a solution to overcome limitations in antibody array development.
To detail the methods for array production and sample detection using this innovative approach.
To expand the capabilities of antibody arrays for comprehensive proteomic analysis.

Main Methods:

Directly labeling all target molecules in biological samples with fluorescent dyes (e.g., Cy3, Cy5).
Utilizing antibody array chips that are pre-composed with capture antibodies.
Applying the fluorescently labeled samples to the antibody array for detection.

Main Results:

Label-based arrays circumvent the need for specific antibody pairs for each target.
This method allows for direct detection of labeled targets on the antibody chip.
Enables higher density arrays and broader proteomic detection compared to traditional methods.

Conclusions:

Label-based array technology offers a viable strategy to enhance proteomic breadth in antibody arrays.
The described methods for array production and sample detection facilitate wider application of this technology.
This approach represents a significant advancement in high-density antibody array development for comprehensive biological analysis.