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Updated: Nov 28, 2025

The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers

The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers

Published on: January 22, 2013

Reverse Phase Protein Arrays.

Justin B Davis¹, Sydney Andes¹, Virginia Espina²

¹Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, VA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|November 25, 2020

Summary

This summary is machine-generated.

Reverse phase protein arrays (RPPA) quantify proteins and modifications. This technology aids biomarker discovery and drug development by immobilizing, detecting, and quantifying proteins on slides using antibody recognition and signal amplification.

Keywords:

Antibody Drug discovery Functional phenotype Nitrocellulose Posttranslational modifications Protein Reverse phase protein array Solid pin

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Last Updated: Nov 28, 2025

The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers

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Published on: January 22, 2013

Identifying Protein-protein Interaction Sites Using Peptide Arrays

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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

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Published on: March 30, 2015

Area of Science:

Biochemistry
Molecular Biology
Biotechnology

Background:

Reverse phase protein arrays (RPPA) are powerful tools for quantifying proteins and their post-translational modifications in biological samples.
RPPA technology supports biomarker discovery, pathway analysis, and understanding drug mechanisms.

Purpose of the Study:

To detail the principles and methodology of Reverse Phase Protein Arrays (RPPA).
To focus on RPPA detection and imaging, specifically using a colorimetric (diaminobenzidine (DAB)) strategy.

Main Methods:

Proteins are immobilized as discrete spots on nitrocellulose-coated slides.
Antibodies are used for specific protein recognition, followed by signal amplification chemistries.
Colorimetric detection using diaminobenzidine (DAB) is employed for imaging and quantification.

Main Results:

RPPA enables precise quantification of protein levels and modifications.
The described method allows for calibrated assays on a single slide, including specimens, controls, and calibrators.
Colorimetric detection provides a reliable method for RPPA imaging and data acquisition.

Conclusions:

RPPA is a versatile technology for proteomic analysis in various biological contexts.
The colorimetric DAB detection strategy offers a robust approach for RPPA data generation.
RPPA facilitates advancements in biomarker discovery and drug development research.