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Related Experiment Video

Updated: Nov 28, 2025

Isolating Lymphocytes from the Mouse Small Intestinal Immune System
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Immune Cell Isolation from Murine Intestine for Antibody Array Analysis.

Joshua A Owens1, Rheinallt M Jones2

  • 1Division of Pediatric Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, USA. rjones5@emory.edu.

Methods in Molecular Biology (Clifton, N.J.)
|November 25, 2020
PubMed
Summary

This study details methods for isolating gut immune cells from mice. These techniques enable functional analysis of immune cell responses to various antigens, crucial for understanding gut health and disease.

Keywords:
Antibody arrayIntestineIntraepithelial lymphocytesLamina propria lymphocytesPeyer’s patch

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Last Updated: Nov 28, 2025

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Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes
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Area of Science:

  • Immunology
  • Gastroenterology
  • Cell Biology

Background:

  • Gut mucosal immune cells are vital for health, responding to microbial and dietary antigens.
  • Understanding host responses requires efficient isolation and functional characterization of intestinal immune cells.

Purpose of the Study:

  • To outline methods for isolating immune cells from murine small and large intestines.
  • To enable functional analysis of these immune cells using antibody array platforms.

Main Methods:

  • Immune cell isolation from murine small intestine.
  • Immune cell isolation from murine large intestine.
  • Functional analysis using antibody array platforms.

Main Results:

  • Established protocols for isolating viable immune cells from the mouse gut.
  • Demonstrated feasibility of functional profiling of isolated intestinal immune cells.

Conclusions:

  • Efficient isolation and functional characterization methods are critical for studying gut mucosal immunity.
  • These methods support research into immune cell roles in health and disease states.