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Updated: Nov 28, 2025

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Phenotypical Characterization and Isolation of Tumor-Derived Mouse Myeloid-Derived Suppressor Cells
Roza Maria Barouni1, Chiara Musiu1, Vincenzo Bronte2
1Department of Medicine, Section of Immunology, University of Verona, Verona, Italy.
Abstract:
Myeloid-derived suppressor cells (MDSCs) are a heterogeneous cell population composed of mature and immature cells of myeloid origin that play a major role in tumor progression by inhibiting the antitumor immune responses mediated by T cells. In this chapter, we describe protocols for isolation, phenotypical and functional evaluation of MDSCs isolated from mouse tumors, with the aim at unifying and standardizing protocols set up by different laboratories.
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