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Updated: Nov 28, 2025

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Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments
Published on: July 10, 2013
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Isolation of Bovine Neutrophils by Fluorescence- and Magnetic-Activated Cell Sorting
Marion Rambault1, Rachana Borkute2, Emilie Doz-Deblauwe1
1INRAE, Univ Tours, ISP, Nouzilly, France.
Methods in Molecular Biology (Clifton, N.J.)
|November 25, 2020
Abstract:
Flow cytometry and magnetic bead technology enable the separation of cell populations with the highest degree of purity. Here, we describe protocols to sort bovine neutrophils from blood, the labeling and sorting, including gating strategies. We also provide advice to preserve neutrophil viability and detail a protocol to measure phagocytosis and oxidative species production.

