Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Nov 28, 2025

Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality

Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality

Published on: April 7, 2023

Probiotic nasal spray development by spray drying.

Katarina Jokicevic¹, Shari Kiekens¹, Eline Byl¹

¹University of Antwerp, Department of Pharmaceutical, Biomedical and Veterinary Sciences, Laboratory of Pharmaceutical Technology and Biopharmacy, Universiteitsplein 1, 2610 Wilrijk, Belgium.

European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V

|November 25, 2020

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

From diversity to dominance: how salt and CO₂ shape LAB-dominated ecosystems in vegetable fermentations.

Microbiology spectrum·2026

Same author

Saccharomyces cerevisiae reduces vulvovaginal candidiasis severity through modulation of fungal pathogenicity and inflammatory responses.

Nature communications·2026

Same author

AIRRWAS integrates TCR-microbiome data to reveal how the gut microbiome shapes the circulating and mucosal T cell repertoire.

Cell reports·2026

Same author

Marine influence on airborne bacterial community composition and predicted functional potential in coastal zones: a case study from Ostend, Belgium.

Scientific reports·2026

Same author

Genome sequence of the stress-tolerant yeast <i>Debaryomyces hansenii</i> DSM 3428.

Microbiology resource announcements·2026

Same author

Practical lessons from microbiome citizen-science projects.

Nature microbiology·2026

This summary is machine-generated.

This study developed a novel probiotic nasal spray using URT-derived lactobacilli. The spray formulation ensures long-term stability and effectiveness in controlling upper respiratory tract infections.

Area of Science:

Microbiology
Pharmaceutical Sciences
Immunology

Background:

Upper respiratory tract (URT) infections are globally prevalent, often caused by viral and bacterial pathogens.
Lactobacilli are increasingly recognized as crucial gatekeepers for maintaining a healthy URT.
Current therapeutic strategies for URT diseases often overlook the potential of probiotics due to formulation challenges.

Purpose of the Study:

To develop and characterize a novel probiotic nasal spray formulation for URT disease prevention and control.
To evaluate the stability, viability, and efficacy of a URT-derived lactobacilli strain produced via spray drying.
To assess the suitability of the probiotic nasal spray for URT applications.

Main Methods:

Spray drying of a novel URT-derived probiotic lactobacilli strain with disaccharide/sugar alcohol and polymer combinations.

Keywords:

Adhesion Antimicrobial activity Lacticaseibacillus Nasal spray Shelf-life Spray drying

More Related Videos

Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods

Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods

Published on: September 10, 2016

Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice

Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice

Published on: February 8, 2016

Related Experiment Videos

Last Updated: Nov 28, 2025

Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality

Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality

Published on: April 7, 2023

Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods

Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods

Published on: September 10, 2016

Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice

Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice

Published on: February 8, 2016

Quantitative and qualitative analysis of spray-dried powders, including long-term stability assessments (>10^9 CFU/g after 28 weeks).

Evaluation of reconstituted formulations for dosage sufficiency, rheological properties, viability, cell morphology post-spraying, adherence to Calu-3 cells, and antimicrobial activity against URT pathogens.

Main Results:

Four spray-dried formulations demonstrated excellent long-term stability (>10^9 CFU/g after 28 weeks).
Reconstituted formulations maintained viability and desirable rheological properties (shear-thinning) for 7-day use and withstood nasal spray application.
The probiotic formulation exhibited high adherence to Calu-3 cells and potent antimicrobial activity against common URT pathogens, unaffected by production or spraying.

Conclusions:

Spray drying is a viable method for producing stable probiotic nasal spray formulations using URT-derived lactobacilli.
The developed probiotic nasal spray demonstrates potential for effective URT disease control and prevention.
The formulation's stability, usability, and antimicrobial properties support its application in managing upper respiratory tract infections.