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Updated: Nov 28, 2025

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

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Long Noncoding RNA PMS2L2 Downregulates miR-24 through Methylation to Suppress Cell Apoptosis in Ulcerative Colitis

Ting Yu1, Fanyu Meng1, Minning Xie1

  • 1Department of TCM Proctlogy, Jinshan Hospital Affiliated to Fudan University, Shanghai, China.

Digestive Diseases (Basel, Switzerland)
|November 25, 2020
PubMed
Abstract

Insights

The long noncoding RNA PMS2L2 is downregulated in ulcerative colitis (UC). PMS2L2 suppresses UC cell apoptosis by inhibiting miR-24 expression through methylation.

Area of Science:

  • Gastroenterology
  • Molecular Biology
  • Inflammation Research

Background:

  • Ulcerative colitis (UC) is a chronic inflammatory bowel disease affecting the colon.
  • The long noncoding RNA (lncRNA) PMS2L2 has demonstrated protective effects against inflammatory injury.

Purpose of the Study:

  • To investigate the role of lncRNA PMS2L2 in the pathogenesis of ulcerative colitis.
  • To elucidate the molecular mechanism by which PMS2L2 influences UC development.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) to measure PMS2L2 and miR-24 expression in UC patients and controls.
  • Bioinformatics, RNA immunoprecipitation, and RNA pull-down assays to confirm PMS2L2-miR-24 interaction.
  • Methylation-specific PCR and Western blotting to assess gene methylation and apoptosis-related protein expression.

Main Results:

  • PMS2L2 expression was significantly downregulated in the plasma of UC patients compared to healthy controls.
  • Overexpression of PMS2L2 inhibited miR-24 expression in human colonic epithelial cells by promoting miR-24 gene methylation.
  • PMS2L2 overexpression suppressed lipopolysaccharide (LPS)-induced apoptosis by upregulating Bcl-2 and downregulating Bax, cleaved-caspase-3, and cleaved-caspase-9.

Conclusions:

  • PMS2L2 plays a protective role in ulcerative colitis by downregulating miR-24 expression via methylation.
  • This mechanism suppresses cell apoptosis, suggesting PMS2L2 as a potential therapeutic target for UC.
Keywords:
Colonic epithelial cellPMS2L2Ulcerative colitismiR-24

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