Related Experiment Video

Updated: Nov 28, 2025

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

9.8K

Genomic retargeting of p53 and CTCF is associated with transcriptional changes during oncogenic HRas-induced

Michal Schwartz1,2, Avital Sarusi Portugez1, Bracha Zukerman Attia1

  • 1The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.

Communications Biology
|November 26, 2020
PubMed

Insights

Cancer development involves changes in gene regulation. This study reveals how transcription factors p53 and CTCF alter gene expression during early oncogene-induced transformation, highlighting the role of regulatory elements in cancer progression.

Area of Science:

  • Genomics
  • Epigenetics
  • Cancer Biology

Background:

  • Gene transcription is controlled by regulatory elements and transcription factors.
  • Changes in chromatin and transcription factor binding at regulatory elements are implicated in cancer.
  • Understanding early oncogene-induced transformation is crucial for cancer research.

Purpose of the Study:

  • To investigate the regulatory network driving transcriptional changes during early oncogene-induced carcinogenic transformation.
  • To identify key transcription factors and regulatory elements involved in this process.

Main Methods:

  • Utilized Hi-C data to analyze spatial coupling between genes and regulatory elements.
  • Characterized transcription factor binding and chromatin accessibility.
  • Focused on early stages of oncogenic HRas-induced transformation in human mammary epithelial cells.

Main Results:

  • Observed spatial coupling between differentially expressed genes and their regulatory elements.
  • Identified p53 and CTCF as key transcription factors influencing early transcriptional alterations.
  • Demonstrated that malignant transcriptional reprogramming is driven by altered chromatin binding of these factors, not expression level changes.

Conclusions:

  • Alterations in the regulatory landscape are critical drivers of oncogene-induced transcriptional reprogramming.
  • The redistribution of transcription factor binding, specifically p53 and CTCF, plays a significant role in early cancer development.
  • This study provides insights into the mechanisms underlying oncogenic transformation.

More Related Videos

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

6.8K
A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells10:13

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

Published on: July 3, 2013

11.4K

Related Concept Videos

Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
10.1K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.9K
The Ras Gene02:38

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
6.8K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.2K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.4K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
8.8K