Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Chromatin Position Affects Gene Expression

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...

Euchromatin

Euchromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...

Euchromatin

Euchromatin

Heterochromatin

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...

Heterochromatin

Heterochromatin

Duplication of Chromatin Structure

Duplication of Chromatin Structure

The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Addressing Biosecurity Barriers in High-Risk Biological Research.

Health security·2026

Same author

CIPHER: An end-to-end framework for designing optimized aggregated spatial transcriptomics experiments.

PLoS computational biology·2026

Same author

CIPHER: An end-to-end framework for designing optimized aggregated spatial transcriptomics experiments.

bioRxiv : the preprint server for biology·2026

Same author

Modeling heterogeneous signaling dynamics of macrophages reveals principles of information transmission in stimulus responses.

Nature communications·2025

Same author

Spatial Single-Cell Mapping of Transcriptional Differences Across Genetic Backgrounds in Mouse Brains.

bioRxiv : the preprint server for biology·2024

Same author

Dynamical and combinatorial coding by MAPK p38 and NFκB in the inflammatory response of macrophages.

Molecular systems biology·2024

Same journal

Turbulent flow in a vortex separator with a directed pipe inlet.

Scientific reports·2026

Same journal

Systematic characteristic evaluation of clay-based cementitious material derived from calcium carbide residue and waste tile powder.

Scientific reports·2026

Same journal

Retraction Note: Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles.

Scientific reports·2026

Same journal

Applying large language models to spam detection in the Kazakh low-resource language setting.

Scientific reports·2026

Same journal

An open-source 3D printing system enabling in-situ freeze-thaw processing of hydrogels.

Scientific reports·2026

Same journal

An enhanced EfficientNet framework for automated waste classification using cosine annealing and label smoothing.

Scientific reports·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 28, 2025

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

Identifying chromatin features that regulate gene expression distribution.

Thanutra Zhang¹, Robert Foreman¹, Roy Wollman^2,3

¹Institute for Quantitative and Computational Biosciences, UCLA, Los Angeles, CA, USA.

Scientific Reports

|November 26, 2020

Summary

This summary is machine-generated.

Chromatin microenvironments significantly influence gene expression variability. This study identifies specific chromatin factors regulating gene expression distributions, advancing our understanding of cellular phenotypes.

More Related Videos

Author Spotlight: Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Author Spotlight: Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Published on: May 12, 2023

Chromatin Immunoprecipitation ChIP using Drosophila tissue

Chromatin Immunoprecipitation ChIP using Drosophila tissue

Published on: March 23, 2012

Related Experiment Videos

Last Updated: Nov 28, 2025

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

Author Spotlight: Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Author Spotlight: Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Published on: May 12, 2023

Chromatin Immunoprecipitation ChIP using Drosophila tissue

Chromatin Immunoprecipitation ChIP using Drosophila tissue

Published on: March 23, 2012

Area of Science:

Molecular Biology
Genomics
Epigenetics

Background:

Gene expression variability, or differences in mRNA per cell, is common across organisms and affects cellular phenotypes.
While chromatin's role in average gene expression is known, its contribution to expression variability remains unclear.
Understanding chromatin's role in gene expression variability is crucial for deciphering cellular function.

Purpose of the Study:

To identify specific aspects of the chromatin microenvironment that contribute to gene expression variability.
To systematically investigate the relationship between chromatin features and gene expression distributions.
To uncover novel factors regulating gene expression variability.

Main Methods:

Utilized a large library of isogenic reporter clones created via DNA barcoding and split-pool decoding.
Systematically investigated randomly integrated expression reporters to assess chromatin's impact.
Mapped reporter expression at diverse genomic loci alongside epigenetic profiles, including transcription factor enrichment and chromatin state proximity.

Main Results:

Identified novel factors within the chromatin microenvironment that regulate gene expression variability.
Demonstrated a link between specific epigenetic profiles and the distribution of gene expression.
Successfully mapped reporter integration sites in a massive, parallel manner.

Conclusions:

Specific chromatin features play a significant role in regulating gene expression variability.
This research provides new insights into the epigenetic regulation of gene expression distributions.
The findings contribute to a deeper understanding of how chromatin structure influences cellular phenotypes.