Synergistic effect of fennel essential oil and hydrogen peroxide on bacterial biofilm

Staphylococcal biofilm formation significantly challenges wound management. The causes of difficult-to-treat wounds are not only methicillin-resistant staphylococci, but also methicillin-sensitive strains with different patterns of resistance. Bacterial biofilm significantly limits the access and activity of antimicrobials used in dermatological infections.

To evaluate the synergistic effect of fennel essential oil (FEO) and H₂O₂ on biofilm formation by Staphylococcus aureus (MSSA and MRSA) reference strains.

Minimum inhibitory concentration (MIC) values were determined for FEO and H₂O₂ against S. aureus reference strains by the broth microdilution method. The combined effects of the FEO and H₂O₂ were calculated and expressed in terms of a fractional inhibitory concentration index (FICI) using the checkerboard method. The FEO composition was analyzed by the GC-MS method. The data were analysed by one-way ANOVA.

Decreased MIC values for FEO combined with H₂O₂ were observed in comparison to FEO itself. The combinations of FEO and H₂O₂ determined synergistic effects on all S. aureus reference strains. Subinhibitory concentration of FEO alone and in combination with 0.5 MIC of H₂O₂ significantly decreased the production of biofilm biomass in S. aureus strains and reduced the metabolic activity of attached cells.

Combination of fennel essential oil containing nearly 80% trans-anethole and H₂O₂ represents a potential for further basic and applied research on wound management.