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Plasma Exosomal MiRNAs Expression Profile in Mesial Temporal Lobe Epilepsy With Hippocampal Sclerosis: Case-Control
Li-Gang Huang1,2, Yun-He Luo2, Ji-Wen Xu3
1Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China.
Background:
To explore an expression profile in plasma exosomal miRNAs of mesial temporal lobe epilepsy with hippocampal sclerosis (mTLE + HS) patients and investigate the associated clinical significance and putative pathways involved.
Methods:
Plasma exosomal miRNAs were measured in six mTLE + HS patients who were confirmed with pre-surgical stereo-electroencephalography and six without hippocampal sclerosis (mTLE-HS) using Illumina HiSeq 2500. Then six dysregulated miRNAs were chosen for validation in an independent sample of 18 mTLE + HS patients and 18 mTLE-HS controls using RT-qPCR. Receiver operating characteristic curve was conducted to evaluate the diagnostic value of miRNAs in HS. Bioinformatic analyses were conducted to reveal in which pathways these miRNAs were involved.
Results:
We revealed that a total of 42 exosomal miRNAs were differentially expressed in mTLE + HS. Among them, 25 were increased and 17 decreased. After validation, hsa-miR-129-5p, -214-3p, -219a-5p, and -34c-5p were confirmed as being upregulated, while hsa-miR-421 and -184 were significantly downregulated in mTLE + HS. Moreover, hsa-miR-184 had the best diagnostic value for discriminating mTLE + HS with 88.9% sensitivity and 83.3% specificity. These six miRNAs regulated several genes from neurotrophin-, hippo-, p53-, TGF- beta-, HIF- 1-, mTOR-related pathways.
Conclusion:
Six miRNAs were dysregulated in mTLE + HS patients and targeted several genes. This result might facilitate pathological mechanistic studies of miRNAs in HS and represent potential diagnostic biomarkers. These provided the rationale for further confirmation studies in larger cohorts of prospective patients.
Insights
Six specific microRNAs (miRNAs) are dysregulated in mesial temporal lobe epilepsy with hippocampal sclerosis (mTLE + HS). These miRNAs show potential as diagnostic biomarkers for mTLE + HS, aiding further research.
Area of Science:
- Neuroscience
- Molecular Biology
- Genetics
Background:
- Mesial temporal lobe epilepsy with hippocampal sclerosis (mTLE + HS) is a common epilepsy syndrome.
- Understanding the molecular mechanisms underlying mTLE + HS is crucial for developing effective treatments.
- Plasma exosomal miRNAs are emerging as potential biomarkers for neurological disorders.
Purpose of the Study:
- To investigate the expression profile of plasma exosomal miRNAs in mTLE + HS patients.
- To identify specific miRNAs associated with mTLE + HS.
- To explore the clinical significance and potential pathways involved in mTLE + HS.
Main Methods:
- Plasma exosomal miRNAs were analyzed using Illumina HiSeq 2500 in mTLE + HS and mTLE-HS patients.
- Six dysregulated miRNAs were validated via RT-qPCR in an independent cohort.
- Bioinformatic analyses identified associated pathways.
Main Results:
- 42 differentially expressed exosomal miRNAs were identified in mTLE + HS patients.
- Six miRNAs (hsa-miR-129-5p, -214-3p, -219a-5p, -34c-5p, -421, and -184) were confirmed as dysregulated.
- hsa-miR-184 demonstrated significant diagnostic value for mTLE + HS (88.9% sensitivity, 83.3% specificity).
Conclusions:
- Six miRNAs are dysregulated in mTLE + HS, suggesting their role in the pathology.
- These miRNAs may serve as potential diagnostic biomarkers for mTLE + HS.
- Further studies in larger cohorts are warranted to confirm these findings.
