Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of ePLAR, the echocardiographic pulmonary to left atrial ratio, in a large cohort with pulmonary hypertension.

European heart journal open·2026
Same author

Author Correction: Enteric neurons increase maternal food intake during reproduction.

Nature·2026
Same author

A multi-readout photonic sensor for rapid diagnosis of Von Willebrand disease.

Npj biosensing·2026
Same author

The association between glucagon-like peptide 1 receptor agonists therapy and outcomes after heart transplant.

The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation·2026
Same author

Echocardiographic hemodynamics correlate with differences in DOAC-specific bleeding and stroke rates in non-valvular atrial fibrillation.

BMC cardiovascular disorders·2025
Same author

Right ventricular strain enhances the REVEAL LITE score for predicting 1-Year mortality in pulmonary hypertension.

The international journal of cardiovascular imaging·2025

Related Experiment Video

Updated: Nov 28, 2025

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS
09:45

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

Published on: June 3, 2011

16.9K

The Drosophila Split Gal4 System for Neural Circuit Mapping.

Haojiang Luan1, Fengqiu Diao1, Robert L Scott1

  • 1Laboratory of Molecular Biology, National Institute of Mental Health, NIH, Bethesda, MD, United States.

Frontiers in Neural Circuits
|November 26, 2020
PubMed
Summary

The Split Gal4 system enables precise genetic targeting of neurons in fruit flies (Drosophila melanogaster). This powerful tool aids in mapping neural circuits and understanding brain function, crucial for neuroscience research.

Keywords:
binary expression systemscell typeconnectomicsgenetic modelsintersectional targeting

More Related Videos

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains
10:13

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains

Published on: November 6, 2017

20.1K
An Explant System for Time-Lapse Imaging Studies of Olfactory Circuit Assembly in Drosophila
07:06

An Explant System for Time-Lapse Imaging Studies of Olfactory Circuit Assembly in Drosophila

Published on: October 13, 2021

2.4K

Related Experiment Videos

Last Updated: Nov 28, 2025

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS
09:45

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

Published on: June 3, 2011

16.9K
Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains
10:13

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains

Published on: November 6, 2017

20.1K
An Explant System for Time-Lapse Imaging Studies of Olfactory Circuit Assembly in Drosophila
07:06

An Explant System for Time-Lapse Imaging Studies of Olfactory Circuit Assembly in Drosophila

Published on: October 13, 2021

2.4K

Area of Science:

  • Neuroscience
  • Genetics
  • Animal Behavior

Background:

  • Understanding complex nervous systems is challenging due to cell diversity and interconnectivity.
  • Genetic techniques for monitoring and manipulating neuronal function in vivo are advancing brain research.
  • Model organisms like Drosophila melanogaster are vital for developing and applying these genetic tools.

Purpose of the Study:

  • To review the Split Gal4 system, a key genetic method for neuronal targeting in Drosophila.
  • To highlight the applications of Split Gal4 in mapping and functionally characterizing neural circuits.
  • To discuss the future potential of Split Gal4 in conjunction with emerging connectomics data.

Main Methods:

  • Utilizing the Split Gal4 system for genetic manipulation and observation of neuronal activity.
  • Employing functional perturbations and behavioral screens to assess circuit roles.
  • Integrating Split Gal4 with connectomics data for neuron characterization.

Main Results:

  • Split Gal4 has been instrumental in characterizing circuits controlling behaviors like grooming, aggression, and mating.
  • The method has enabled comprehensive mapping of key brain regions, including the central complex, lateral horn, and mushroom body.
  • Split Gal4 is poised to aid in characterizing neurons based on connectivity data from Drosophila brains.

Conclusions:

  • The Split Gal4 system is a powerful and increasingly important tool in Drosophila neuroscience.
  • Its application facilitates detailed mapping and functional analysis of neural circuits.
  • Future developments promise expanded utility, especially with advances in connectomics.