Biomass-Derived Multilayer-Structured Microparticles for Accelerated Hemostasis and Bone Repair

Area of Science:

• Biomaterials Science
• Biomedical Engineering
• Nanotechnology

Background:

• Developing sustainable, safe, and bioactive biomedical materials is crucial for clinical applications.
• Bone defects with intractable bleeding require advanced treatment solutions.
• Biomass-derived materials offer potential for sustainable biomedical applications.

Purpose of the Study:

• To construct biomass-derived multilayer-structured absorbable microparticles (MQ T) for treating bone defects with intractable bleeding.
• To evaluate the biosafety, bioactivity, and hemostatic performance of these microparticles.
• To demonstrate their potential for promoting bone repair and reducing inflammation.

Main Methods:

• Layer-by-layer assembly of quaternized starch (Q+) and tannic acid onto microporous starch microparticles.
• Characterization of microparticle properties including degradability, cytotoxicity, and blood compatibility.
• In vivo evaluation in a mouse cancellous bone defect model and a beagle proof-of-concept study.

Main Results:

• MQ T microparticles demonstrated efficient degradability, low cytotoxicity, and good blood compatibility.
• MQ2T2 microparticles, with an outermost polyphenol layer, showed superior platelet adhesion, red blood cell aggregation, and hemostatic performance.
• In vivo studies confirmed favorable hemostasis, low inflammation, high biodegradability, and promoted bone repair with MQ2T2.

Conclusions:

• Biomass-derived multilayer microparticles are promising for safe and effective treatment of bone defects with intractable bleeding.
• The MQ2T2 formulation exhibits excellent hemostatic properties and promotes bone regeneration.
• These materials represent a significant advancement in sustainable biomedical solutions for clinical use.