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Cyclin G2 Inhibits Oral Squamous Cell Carcinoma Growth and Metastasis by Binding to IGFBP3 and Regulating the

Danning Wang1, Jinlan Gao1, Chenyang Zhao1

  • 1The Research Center for Medical Genomics, Key Laboratory of Cell Biology, Key Laboratory of Medical Cell Biology, Ministry of Education, School of Life Sciences, China Medical University, Shenyang, China.

Frontiers in Oncology
|November 26, 2020
PubMed

Insights

Cyclin G2 acts as a tumor suppressor in oral squamous cell carcinoma (OSCC). Overexpression of cyclin G2 inhibits OSCC proliferation and metastasis by interacting with IGFBP3, suggesting it as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Cyclin G2 is a cell cycle protein with known tumor suppressor roles.
  • Its specific functions and regulatory mechanisms in oral cancers remain largely unelucidated.
  • Oral squamous cell carcinoma (OSCC) represents a significant global health challenge.

Purpose of the Study:

  • To investigate the role and underlying mechanisms of cyclin G2 in oral squamous cell carcinoma.
  • To determine the prognostic significance of cyclin G2 expression in OSCC patients.

Main Methods:

  • Analysis of cyclin G2 expression in 80 OSCC patient samples.
  • In vitro assays including cell counting Kit-8, flow cytometry, wound-healing, and cell invasion assays.
  • Western blotting and real-time PCR to assess protein and gene expression.
  • Orthotopic nude mice model for in vivo validation.

Main Results:

  • Low cyclin G2 expression in OSCC is linked to advanced clinical staging and poorer prognosis.
  • Overexpression of cyclin G2 significantly inhibited OSCC cell proliferation, metastasis, and induced G1/S cell cycle arrest.
  • Cyclin G2 was found to inhibit OSCC progression by binding to insulin-like growth factor binding protein 3 (IGFBP3) and modulating the FAK-SRC-STAT pathway.

Conclusions:

  • Cyclin G2 exhibits a tumor-suppressive role in oral squamous cell carcinoma.
  • The interaction between cyclin G2 and IGFBP3 is crucial for inhibiting OSCC growth and metastasis.
  • Cyclin G2 represents a promising novel therapeutic target for oral cancer treatment.
Keywords:
c-Srccell cycle protein cyclin G2focal adhesion kinaseinsulin-like growth factor binding protein 3integrinoral squamous cell carcinomatumor suppressor

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