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Abundant Neural circRNA Cdr1as Is Not Indispensable for Retina Maintenance.

Xue-Jiao Chen1, Meng-Lan Li1, Ya-Han Wang1

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Circular RNA Cdr1as is abundant in retinas. Its absence in knockout mice caused mild vision changes, suggesting Cdr1as is not essential for retinal maintenance.

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Area of Science:

  • Ophthalmology
  • Genetics
  • Molecular Biology

Background:

  • Circular RNA Cdr1as is highly abundant in vertebrate retinas.
  • The specific function of Cdr1as in retinal biology is currently unknown.

Purpose of the Study:

  • To investigate the role of Cdr1as in the retina by generating a knockout mouse model.
  • To determine the consequences of Cdr1as loss of function on retinal physiology and visual performance.

Main Methods:

  • Generation of Cdr1as knockout (KO) mice using CRISPR/Cas9 technology.
  • In situ hybridization (ISH) to determine Cdr1as expression patterns in the retina.
  • Ocular examinations and electrophysiological tests (b-wave amplitude) on KO and wild-type (WT) mice.
  • Small RNA sequencing to analyze microRNA and gene expression changes.

Main Results:

  • Cdr1as is primarily expressed in the inner retina.
  • Cdr1as KO mice exhibited increased b-wave amplitude in photopic electroretinography.
  • KO mice showed reduced vision contrast sensitivity compared to WT controls.
  • Downregulation of miR-7 and upregulation of its target genes were observed in KO retinas.

Conclusions:

  • Cdr1as ablation results in mild retinal functional deficits in mice.
  • The study indicates that Cdr1as is not indispensable for retinal development and maintenance.
  • These findings provide novel insights into the function of circular RNAs in the visual system.