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Updated: Nov 28, 2025

Cell Population Analyses During Skin Carcinogenesis
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Cell Population Analyses During Skin Carcinogenesis

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High expression of disabled homolog 2-interacting protein contributes to tumor development and proliferation in

Shao-Ping Yuan1,2, Chang-Xing Li3, Si Qin1,2

  • 1The Second School of Clinical Medicine, Southern Medical University, Guangzhou, China.

Annals of Translational Medicine
|November 26, 2020
PubMed
Abstract

Insights

High expression of Disabled homolog 2-interacting protein (DAB2IP) is linked to increased cutaneous squamous cell carcinoma (cSCC) development and proliferation. Knocking down DAB2IP inhibits cSCC cell viability and invasion, suggesting its potential as a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology

Background:

  • Disabled homolog 2-interacting protein (DAB2IP), a Ras GTPase-activating protein, is frequently downregulated in various cancers.
  • DAB2IP depletion is implicated in tumor cell proliferation, apoptosis, metastasis, and epithelial-mesenchymal transition.

Purpose of the Study:

  • To investigate the role of DAB2IP in cutaneous squamous cell carcinoma (cSCC).
  • To establish a theoretical foundation for cSCC diagnosis and targeted therapy.

Main Methods:

  • Immunohistochemistry was used to analyze DAB2IP expression in cSCC tissues.
  • In vitro experiments using SCL-1 cells with genetically reduced DAB2IP expression (via shRNA) assessed cell behavior.
  • Techniques included RT-qPCR, MTT assay, colony formation, cell cycle analysis, apoptosis assays, Transwell, and wound-healing assays.

Main Results:

  • DAB2IP expression was higher in cSCC tissues compared to soft fibroma.
  • Elevated DAB2IP levels correlated with malignancy grade and tumor infiltration depth.
  • Knockdown of DAB2IP suppressed SCL-1 cell viability and invasion in vitro.

Conclusions:

  • High DAB2IP expression may promote the development and proliferation of cSCC.
  • DAB2IP represents a potential biomarker and therapeutic target for cSCC.
Keywords:
Cutaneous squamous cell carcinoma (cSCC)SCL-1disabled homolog 2-interacting protein (DAB2IP)immunohistochemistryinvasionproliferation

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