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Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
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Upconversion Fluorescence in Naturally Occurring Calcium Fluoride.

Jillian Elizabeth Moffatt1,2, Thomas Bede Payten1,2, Georgios Tsiminis1,2,3

  • 1Institute for Photonics and Advanced Sensing, School of Physical Sciences, The University of Adelaide, Adelaide, Australia.

Applied Spectroscopy
|November 26, 2020
PubMed
Summary
This summary is machine-generated.

Upconversion fluorescence offers a new method for real-time fluorine detection in mineral processing. This technique enhances detection of fluorite (CaF2) by analyzing rare earth inclusions, improving ore processing efficiency.

Keywords:
Fluoritecalcium fluoridefluorescencefluorine mineral sensingluminescencemineral sensingupconversion

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Area of Science:

  • Mineralogy
  • Geochemistry
  • Analytical Chemistry

Background:

  • Fluorine negatively impacts mineral processing, causing issues in leaching and smelting.
  • Current fluorine detection methods are offline, limiting real-time process control.
  • Accurate, real-time fluorine monitoring is crucial for protecting equipment and optimizing mineral production.

Purpose of the Study:

  • To develop a novel, real-time sensing modality for fluorine-bearing minerals, specifically fluorite (CaF2).
  • To overcome limitations of traditional fluorescence methods in complex mineral matrices.
  • To improve efficiency and decision-making in mine production through enhanced fluorine detection.

Main Methods:

  • Utilized upconversion fluorescence spectroscopy for mineral analysis.
  • Employed near-infrared excitation to analyze natural fluorite samples.
  • Investigated rare earth inclusions within fluorite as indicators for upconversion fluorescence.

Main Results:

  • Upconversion fluorescence provided highly host-specific signals, minimizing interference in complex samples.
  • Detected upconversion fluorescence in fluorite samples with rare earth concentrations as low as one part per million.
  • Demonstrated the potential of upconversion fluorescence for sensitive and specific fluorite detection.

Conclusions:

  • Upconversion fluorescence is a promising sensing modality for real-time fluorite monitoring in mineral processing.
  • This technique can significantly improve detection thresholds and reduce spurious signals.
  • Enables enhanced efficiency in mineral processing decisions by providing real-time fluorine concentration data.