Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A mild method for decomplementation of human sera.

T Barkas, W J Irvine

    Clinical and Experimental Immunology
    |August 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Pillars Article: Antibodies to Pancreatic Islet Cells in Insulin-dependent Diabetics with Coexistent Autoimmune Disease. <i>Lancet</i>. 1974. 304: 1529-1531.

    Journal of immunology (Baltimore, Md. : 1950)·2017
    Same author

    Antibodies begin to count in the neurology clinic.

    Immunology today·2014
    Same author

    Autoimmunity in endocrine disease.

    Proceedings of the Royal Society of Medicine·2010
    Same author

    Studies on the Cytotoxic Factor in Thyroid Disease.

    British medical journal·2010
    Same author

    Antigenic differences between a proteolipid and a proteodetergent from Torpedo electroplax having similar cholinergic binding properties.

    Neurochemistry international·2010
    Same author

    Anatomy of the antigenic structure of a large membrane autoantigen, the muscle-type nicotinic acetylcholine receptor.

    Immunological reviews·1998
    Same journal

    Could we discuss the molecular signature of immune dysregulation?

    Clinical and experimental immunology·2026
    Same journal

    The many faces of cytokine storm syndrome: immunopathogenic mechanisms and clinical implications for a better patient management.

    Clinical and experimental immunology·2026
    Same journal

    PD-1 Engagement and Circulating Th17 Effector Activity During Secukinumab Treatment in Plaque Psoriasis.

    Clinical and experimental immunology·2026
    Same journal

    Henoch-Schoenlein purpura (HSP) like lesions in IL12RB1 and IL12B defects - A multi-centric experience from India.

    Clinical and experimental immunology·2026
    Same journal

    Case Series on the Efficacy of Daratumumab in Treating Patients with Anti-Interferon-Gamma Autoantibodies.

    Clinical and experimental immunology·2026
    Same journal

    Deleterious germline CARD11 gain-of-function variants alter human B-cell and CD4+ T-cell differentiation and function.

    Clinical and experimental immunology·2026
    See all related articles

    A new, mild method effectively removes complement from human serum using mercaptoethanol. This treatment minimally impacts Immunoglobulin M (IgM) antibodies, preserving their function.

    Area of Science:

    • Immunology
    • Biochemistry

    Background:

    • Complement system plays a crucial role in innate and adaptive immunity.
    • Complement activation can interfere with certain immunological assays and research.
    • Need for mild methods to preserve antibody integrity during serum processing.

    Purpose of the Study:

    • To describe a mild method for human serum decomplementation.
    • To evaluate the impact of this method on Immunoglobulin M (IgM) antibodies.

    Main Methods:

    • Human serum was treated with mercaptoethanol.
    • The decomplementation efficiency was assessed.
    • The effect on IgM antibody integrity was analyzed.

    Main Results:

    • Mercaptoethanol provided a mild method for serum decomplementation.

    Related Experiment Videos

  • The treatment showed a slight effect on IgM antibody levels.
  • Serum complement activity was significantly reduced.

    • Conclusions:

    • Mercaptoethanol offers a gentle approach for human serum decomplementation.
    • This method is suitable for applications where IgM antibody function is important.
    • Further studies may explore effects on other antibody isotypes.