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Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
Published on: July 28, 2023
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Epigenetic modification mechanisms involved in keloid: current status and prospect
Wenchang Lv1, Yuping Ren1, Kai Hou1
1Department of Plastic and Aesthetic Surgery, NO 1095 Jiefang Avenue, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (HUST), Wuhan, 430000, Hubei, China.
Clinical Epigenetics
|November 27, 2020
Summary
Epigenetic modifications, including DNA methylation and non-coding RNAs, drive keloid fibroblast behavior. Understanding these epigenetic changes offers new therapeutic targets for keloid treatment.
Area of Science:
- Dermatology
- Molecular Biology
- Epigenetics
Background:
- Keloids are benign skin tumors resulting from excessive fibroblast proliferation and extracellular matrix accumulation.
- Current keloid therapies show limited efficacy, necessitating novel treatment strategies.
- Epigenetics offers a bridge between genetic and environmental factors in disease development.
Purpose of the Study:
- To review and summarize current research on epigenetic modifications in keloid pathogenesis.
- To investigate the expression profiles and mechanisms of epigenetic alterations in keloid progression.
- To explore the role of epigenetic modifications in keloid-derived fibroblast behavior.
Main Methods:
- Comprehensive review of clinical and experimental studies on keloid epigenetics.
- Analysis of DNA methylation, histone modification, and non-coding RNA (ncRNA) expression in keloids.
- Examination of how epigenetic changes affect fibroblast proliferation, migration, apoptosis, and differentiation.
Main Results:
- Abnormal epigenetic modifications, including DNA methylation, histone modification, and ncRNAs (miRNA, lncRNA, circRNA), are critical in keloid development.
- These epigenetic alterations significantly influence keloid-derived fibroblast functions.
- Evidence highlights the role of epigenetics in keloid pathogenesis and fibroblast dysregulation.
Conclusions:
- Epigenetic modifications are key drivers in keloid formation and progression.
- Targeting epigenetic mechanisms presents a promising avenue for novel keloid diagnostics and therapeutics.
- Further research into keloid epigenetics can uncover new insights into disease etiology and treatment.
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