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LncRNA NEAT1 Promotes the Progression of Gastric Cancer Through Modifying the miR-1224-5p/RSF1 Signaling Axis

Luoluo Yang1, Min Wang2, Ping He1

  • 1Department of Gastroenterology, First Hospital of Jilin University, Changchun 130021, Jilin, People's Republic of China.

Cancer Management and Research
|November 27, 2020
PubMed
Abstract

Insights

Nuclear paraspeckle assembly transcript 1 (NEAT1) promotes gastric cancer progression by sponging miR-1224-5p, leading to increased remodeling and spacing factor 1 (RSF1) expression. This finding offers new avenues for gastric cancer diagnosis and therapy.

Area of Science:

  • Molecular Oncology
  • Epigenetics
  • Cancer Biology

Background:

  • Advanced gastric cancer presents significant therapeutic challenges.
  • Long non-coding RNAs (lncRNAs) are implicated in gastric cancer, but specific roles like NEAT1 remain underexplored.

Purpose of the Study:

  • To investigate the functional role and molecular mechanism of NEAT1 in gastric cancer.
  • To elucidate the regulatory relationship between NEAT1, miR-1224-5p, and RSF1 in gastric cancer progression.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC) to assess NEAT1, miR-1224-5p, and RSF1 expression.
  • Bioinformatics analysis (ENCORI) for predicting binding sites.
  • Cell proliferation (CCK-8), migration (wound healing, Transwell), and interaction (luciferase) assays to validate molecular mechanisms.

Main Results:

  • NEAT1 and RSF1 were highly expressed, while miR-1224-5p was decreased in gastric cancer tissues.
  • NEAT1 upregulation enhanced gastric cancer cell proliferation and migration.
  • NEAT1 acts as a molecular sponge for miR-1224-5p, inhibiting its binding to RSF1 and promoting cancer cell malignancy.

Conclusions:

  • NEAT1 regulates RSF1 expression via competitive sponging of miR-1224-5p.
  • This regulatory axis contributes to gastric cancer progression.
  • Findings provide novel insights for gastric cancer diagnosis and therapeutic strategies.
Keywords:
competes endogenous RNAsgastric cancerlong non-coding RNAsmicroRNAsnuclear paraspeckle assembly transcript 1remodeling and spacing factor 1

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