Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bovine interleukin 2: cloning, high level expression, and purification.

P E Baker1

  • 1Immunex Corporation, Seattle, WA.

Veterinary Immunology and Immunopathology
|December 1, 1987
PubMed
Summary

Researchers isolated bovine interleukin-2 (IL2) using a human IL2 probe. Large quantities of purified bovine IL2 were produced in yeast, enabling further study of its therapeutic potential in cattle.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Helicobacter pylori with a truncated lipopolysaccharide O chain fails to induce gastritis in SCID mice injected with splenocytes from wild-type C57BL/6J mice.

Infection and immunity·2004
Same author

California residential air exchange rates and residence volumes.

Journal of exposure analysis and environmental epidemiology·1996
Same author

Regulation of murine B-cell growth in vitro by infection with a retroviral vector containing the murine IL-1R.

Annals of the New York Academy of Sciences·1992
Same author

Experimental autoimmune encephalomyelitis is exacerbated by IL-1 alpha and suppressed by soluble IL-1 receptor.

Journal of immunology (Baltimore, Md. : 1950)·1991
Same author

Bovine recombinant granulocyte-macrophage colony-stimulating factor enhancement of bovine neutrophil functions in vitro.

American journal of veterinary research·1990
Same author

Selective alteration of bovine neutrophil responses by recombinant bovine interleukin-1 beta.

Veterinary immunology and immunopathology·1990

Area of Science:

  • Veterinary Immunology
  • Molecular Biology
  • Biotechnology

Background:

  • Interleukin-2 (IL2) is a critical cytokine for immune responses.
  • Bovine IL2 (bIL2) has potential therapeutic applications in cattle.
  • Efficient production of bIL2 is necessary for further research.

Purpose of the Study:

  • To isolate and express bovine IL2 (bIL2).
  • To develop a scalable purification method for recombinant bIL2 (rbIL2).
  • To produce sufficient quantities of rbIL2 for future studies on its utility in cattle.

Main Methods:

  • Utilized a human IL2 probe to identify bovine IL2 sequences from a lymph node cDNA library.
  • Expressed bovine IL2 in bacterial and yeast systems.
  • Developed a rapid, two-step purification process for yeast-secreted rbIL2.

Related Experiment Videos

Main Results:

  • Successfully isolated bovine IL2 sequences.
  • Achieved expression of bovine IL2 in both bacteria and yeast.
  • Obtained over 20 mg/l of homogenous, yeast-secreted bovine rbIL2 using the developed purification scheme.

Conclusions:

  • A scalable method for producing homogenous bovine rbIL2 was established.
  • The availability of substantial amounts of rbIL2 facilitates investigation into its therapeutic and prophylactic uses in cattle.
  • This work provides a foundation for exploring IL2-based immunotherapies in veterinary medicine.