Effects of exogenous vitamin B₁₂ on nutrient removal and protein expression of algal-bacterial consortium

Chlorella vulgaris and Bacillus licheniformis consortium was added to synthetic wastewater with exogenous vitamin B₁₂. In the presence of 100 ng/L vitamin B₁₂, removal efficiencies of TN, NH₃-N, PO₄^3-P, and COD were 80.1%, 76.8%, 87.9%, and 76.7%, respectively. The functional groups on the cell surface of the consortium, including -NH, -CH₃, C=O, C=C, and P-O-C, increased with 100 ng/L vitamin B₁₂. These functional groups improved the biological adsorption of the consortium; however, higher concentrations of vitamin B₁₂ resulted in an occlusion of the functional groups. Furthermore, there were 5 significantly enriched protein pathways, namely carbon fixation in photosynthetic organisms; amino acid metabolic pathways; the pathway of one carbon pool by folate; nitrogen metabolism; and photosynthesis. Most proteins in these pathways were upregulated, which enhanced carbon fixation and photosynthesis in the algal cells. Simultaneously, B₁₂ promoted significant upregulation of proteins associated with the quorum-sensing pathway, which promoted the interaction between algae and bacteria.