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Related Concept Videos

Chromatography: Introduction01:10

Chromatography: Introduction

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Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
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Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
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Chromatographic Methods: Terminology01:18

Chromatographic Methods: Terminology

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Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
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Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

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Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
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Thin-Layer Chromatography (TLC): Overview01:11

Thin-Layer Chromatography (TLC): Overview

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Thin-layer chromatography (TLC) is a chromatography technique that separates compounds based on their polarity. TLC typically uses polar silica gel, a form of silicon dioxide, as the stationary phase. The silica gel contains hydroxyl (OH) groups on its surface, which form hydrogen bonds with polar compounds, influencing their adhesion to the stationary phase.
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...
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Principles Of Column Chromatography01:13

Principles Of Column Chromatography

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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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Updated: Nov 28, 2025

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis
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Application of a stepwise frontal analysis method in cell membrane chromatography.

Xiaoshuang He1, Yue Sui2, Sicen Wang3

  • 1Department of Pharmacy, Ruijin Hospital Affiliated to School of Medicine, Shanghai Jiaotong University, Shanghai, 200025, China; School of Pharmacy, Xi'an Jiaotong University, Xi'an, 710061, China.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|November 27, 2020
PubMed
Summary

A new cell membrane chromatography method combined with stepwise frontal analysis efficiently evaluates drug-receptor binding affinity. This approach offers a faster, less wasteful alternative for characterizing drug interactions with membrane receptors.

Keywords:
Cell membrane chromatographyEquilibrium dissociation constant (K(d))Stepwise frontal analysis

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Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
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Area of Science:

  • Biochemistry
  • Pharmacology
  • Analytical Chemistry

Background:

  • Bio-affinity chromatography is crucial for studying drug-receptor interactions.
  • Traditional methods for evaluating drug-receptor binding can be time-consuming and resource-intensive.

Purpose of the Study:

  • To develop and validate a novel cell membrane chromatography (CMC) method coupled with stepwise frontal analysis (SFA).
  • To investigate the binding affinity of model drugs to the alpha 1A adrenergic receptor (α1A AR).
  • To establish a more efficient method for determining drug-receptor binding characteristics.

Main Methods:

  • Development of a high-expression α1A AR cell membrane chromatography (CMC) method.
  • Application of stepwise frontal analysis (SFA) based on frontal analysis (FA).
  • Determination of equilibrium dissociation constant (Kd) values for drug-receptor interactions.

Main Results:

  • The CMC/SFA method demonstrated high consistency with traditional FA for determining Kd values.
  • Equilibrium dissociation constant (Kd) values for three model α1A AR-binding drugs were successfully determined.
  • The developed method proved effective in evaluating drug-receptor binding characteristics.

Conclusions:

  • The CMC/SFA method is a time-saving and less wasteful alternative to traditional techniques.
  • This method is suitable for studying interactions between drugs and membrane receptors.
  • The study validates a novel approach for drug-receptor affinity assessment.