Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bioremediation

Bioremediation

Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.

Environmental Applications of Microorganisms

Environmental Applications of Microorganisms

Microorganisms play a pivotal role in maintaining ecosystem balance by recycling essential elements such as carbon, nitrogen, and phosphorus, as well as supporting processes like bioremediation, wastewater treatment, and biofuel production.Microbes in Elemental CyclesIn the carbon cycle, microorganisms decompose organic matter, releasing carbon dioxide via aerobic respiration. This carbon dioxide is subsequently used by photosynthetic organisms to synthesize organic compounds, closing the...

Metabolism of Chemolithotrophs

Metabolism of Chemolithotrophs

Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.

Inorganic Nitrogen Assimilation

Inorganic Nitrogen Assimilation

Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...

Overview of Nitrogen Metabolism

Overview of Nitrogen Metabolism

Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of ammonia, ammonium ions, nitrate, nitrite, or nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...

The Nitrogen Cycle

The Nitrogen Cycle

Nitrogen atoms, present in all proteins and DNA, are recycled between abiotic and biotic components of the ecosystem. However, the primary form of nitrogen on Earth is nitrogen gas, which cannot be used by most animals and plants. Thus, nitrogen gas must first be converted into a usable form by nitrogen-fixing bacteria before it can be cycled through other living organisms. The use of nitrogen-containing fertilizers and animal waste products in human agriculture has greatly influenced the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Diagnostic pitfalls and molecular insights in a rare RUNX1::TACC1-positive AML with t(8;21)(p11;q22).

Journal of hematopathology·2026

Same author

Pyrogenic Carbon Structures Determined Electron Transfer Mechanism Enhancing Iron Autotrophic Denitrification: Role of Oxygen-Containing Functional Groups and Graphitic Structures.

Environmental science & technology·2026

Same author

Plasma proteome-metabolome signatures enable non-invasive early detection and lymph node risk stratification in breast cancer.

Molecular cancer·2026

Same author

Corrigendum to "POU2F3 in Small Cell Lung Cancer (SCLC): Diagnostic Utility in Neuroendocrine-Low/Negative SCLC and Discrimination From Other Thoracic Malignancies and Other Small Blue Round Cell Tumors" [Laboratory Investigation 2026;106(6):106124].

Laboratory investigation; a journal of technical methods and pathology·2026

Same author

Deep learning-based Breast Imaging Reporting and Data System classification and establishment of diagnostic model in breast cancer diagnosis with automated breast ultrasound.

Quantitative imaging in medicine and surgery·2026

Same author

Docosahexaenoic acid alleviates Ornidazole toxicity related to spermatogenic dysfunction by modulating PPARγ-dependent ER-mitochondrial uncoupling.

Science China. Life sciences·2026

Same journal

Microbiota under bloom stress: A review and meta-analysis of bloom-associated cyanopeptides, environmental stressors, and microbial shifts in free-living and host gut microbiomes.

The Science of the total environment·2026

Same journal

Environmental associations in spectral index-based soil salinity detection: A multi-region and multi-sensor assessment.

The Science of the total environment·2026

Same journal

Decoding seasonal urban heat dynamics at neighborhood-scale using explainable deep learning for climate-resilient, digital twin-ready green planning.

The Science of the total environment·2026

Same journal

The effects of microcystin-LR and its location within an environmental pool on rusty crayfish (Faxonius rusticus) behavior and physiology.

The Science of the total environment·2026

Same journal

An advanced hydrological approach for the characterization of the Water Scarcity Footprint at the sub-basin level.

The Science of the total environment·2026

Same journal

Irrigation management and groundwater recharge in Mediterranean intermontane basins: a multi-method evaluation of agricultural controls and interbasin variability.

The Science of the total environment·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 28, 2025

Mesocosm-Scale Constructed Wetland Design for Wastewater Treatment

Mesocosm-Scale Constructed Wetland Design for Wastewater Treatment

Published on: May 2, 2025

Nitrogen removal through collaborative microbial pathways in tidal flow constructed wetlands.

Manping Zhang¹, Jung-Chen Huang¹, Shanshan Sun¹

¹School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, PR China.

The Science of the Total Environment

|November 28, 2020

Summary

This summary is machine-generated.

This study reveals key nitrogen loss pathways in tidal wetlands, highlighting nitrification and denitrification as major processes. Understanding these nitrogen cycles is crucial for effective wetland wastewater treatment.

Keywords:

Anammox Artificial tidal wetlands DNRA Denitrification Nitrification Nitrogen removal

More Related Videos

Visualizing Methane-Cycling Microbial Dynamics in Coastal Wetlands

Visualizing Methane-Cycling Microbial Dynamics in Coastal Wetlands

Published on: January 31, 2025

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Published on: October 15, 2015

Related Experiment Videos

Last Updated: Nov 28, 2025

Mesocosm-Scale Constructed Wetland Design for Wastewater Treatment

Mesocosm-Scale Constructed Wetland Design for Wastewater Treatment

Published on: May 2, 2025

Visualizing Methane-Cycling Microbial Dynamics in Coastal Wetlands

Visualizing Methane-Cycling Microbial Dynamics in Coastal Wetlands

Published on: January 31, 2025

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Published on: October 15, 2015

Area of Science:

Environmental Science
Microbiology
Ecology

Background:

Constructed wetlands effectively remove nitrogen, but nitrogen loss pathways in tidal systems remain unclear.
Tidal flow constructed wetlands (TF-CWs) present unique conditions for nitrogen cycling due to fluctuating water levels and salinity.

Purpose of the Study:

To investigate nitrogen loss pathways, including dissimilatory nitrate reduction to ammonium (DNRA), denitrification, nitrification, and anaerobic ammonium oxidation (anammox).
To quantify the contributions of these pathways to overall nitrogen removal in two tidal wetland mesocosms (planted and unplanted).
To identify key environmental factors regulating these nitrogen transformation processes.

Main Methods:

Molecular and stable isotope analyses were employed to assess microbial community potential and nitrogen transformation rates.
Two tidal wetland mesocosms, one planted with Phragmites australis (PA) and one unplanted (NP), were used.
Mesocosms treated Yangtze River Estuary water, and nitrogen removal efficiencies were measured.

Main Results:

The mesocosms removed approximately 22.6% of total nitrogen (TN), with the planted mesocosm (PA) showing higher efficiency than the unplanted (NP).
Nitrification and denitrification were the dominant nitrogen transformation processes, contributing significantly to nitrogen removal.
Specific nitrogen transformation rates varied, with nitrification and denitrification showing higher activity in the PA mesocosm.

Conclusions:

Nitrification, denitrification, anammox, and DNRA collectively contribute to nitrogen removal in tidal flow constructed wetlands.
Planted wetlands enhance nitrogen removal efficiency compared to unplanted systems.
Ammonium, nitrate, and nitrite concentrations are critical regulators of these nitrogen cycling pathways, offering insights for optimizing wetland design and function.