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Laryngeal Reconstruction Using Tissue-Engineered Implants in Pigs: A Pilot Study.

Sarah Brookes¹, Lujuan Zhang², Theodore J Puls³

¹Department of Basic Medical Sciences, Purdue University, West Lafayette, Indiana, U.S.A.

The Laryngoscope

|November 28, 2020

Summary

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This summary is machine-generated.

This study demonstrates a novel tissue-engineered implant for laryngeal reconstruction after hemilaryngectomy in pigs. The implant successfully restored dynamic laryngeal function, offering hope for patients needing voice restoration.

Area of Science:

Regenerative Medicine
Biomaterials Science
Otolaryngology

Background:

Current treatments for hemilaryngectomy do not restore dynamic laryngeal function.
Previous research showed successful restoration in a rat model.
Scalability to larger animal models is crucial for clinical translation.

Purpose of the Study:

To assess the feasibility of a tissue-engineered implant for laryngeal reconstruction in a porcine model.
To evaluate the restoration of dynamic laryngeal function after partial laryngectomy.
To demonstrate the scalability of a previously successful technique.

Main Methods:

Three Yucatan minipigs underwent left partial laryngectomy.
Customized, multi-layered tissue-engineered implants using autologous muscle progenitor cells (MPCs) and adipose stem cells (ASCs) were created.

Keywords:

Larynx adipose stem cell cartilage chondrocytes collagen oligomer tissue engineering

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Implants consisted of collagen-based mucosal, muscle, and cartilage layers, with MPCs differentiated to express motor endplates.

Main Results:

Pigs exhibited normal weight gain, no aspiration, and audible phonation post-surgery.
Video laryngoscopy confirmed progressive healing, vascularization, and re-epithelialization by 4 weeks.
Histology revealed no immune response, complete integration, and nerve/vascular ingrowth into the implants.

Conclusions:

This pilot study is the first to successfully repair a transmural vertical partial laryngectomy using a customized, multi-layered, stem cell-derived tissue-engineered implant.
The findings support the potential of this technique for restoring laryngeal function.
The porcine model demonstrates the scalability of this regenerative approach for clinical application.