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Measurement of testosterone in seminal plasma, saliva and serum by solid-phase enzymeimmunoassay
C C Shieh1, S C Chang, C R Tzeng
1Department of Medicine, Veterans General Hospital, Taipei, Taiwan R.O.C.
Andrologia
|November 1, 1987
Abstract:
When testosterone concentrations in serum, saliva and seminal plasma were measured with a solid-phase enzymeimmunoassay in which antitestosterone antibody was bound to CNBr-Sepharose-4B, we found the ratios of testosterone in saliva and seminal plasma to that in serum were 2.5 +/- 0.2% and 5.19 +/- 0.42%. Testosterone level in saliva could be an index of free serum testosterone. In addition, we found a correlation between sperm motility and testosterone level in seminal plasma when sperm motility was measured with a transmembrane migration method. This enzymeimmunoassay could be applied to clinical studies of testosterone.