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Related Experiment Videos

Maximizing productivity in an immobilized cell reactor.

J L Vega1, E C Clausen, J L Gaddy

  • 1Department of Chemical Engineering, University of Arkansas, Fayetteville 72701.

Annals of the New York Academy of Sciences
|January 1, 1987
PubMed
Summary

This study demonstrates a stable vertical immobilized cell reactor using Saccharomyces cerevisiae for high-efficiency glucose conversion. Optimal productivity is achieved with specific substrate concentrations and packing materials, outperforming horizontal configurations.

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Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Yeast Fermentation

Background:

  • Immobilized cell reactors (ICRs) offer high cell concentrations and dilution rates.
  • Saccharomyces cerevisiae is a key microorganism for industrial fermentation processes.
  • Reactor stability and productivity are critical for efficient bioprocessing.

Purpose of the Study:

  • To evaluate the performance of a vertical immobilized cell reactor (ICR) using Saccharomyces cerevisiae.
  • To investigate the impact of packing materials and operational parameters on reactor efficiency.
  • To compare the performance of vertical and horizontal ICR configurations.

Main Methods:

  • Employing Saccharomyces cerevisiae cross-linked to a gelatin support with glutaraldehyde in a vertical reactor.

Related Experiment Videos

  • Analyzing glucose concentration profiles and correlating them with residence time and inlet concentration.
  • Comparing various packing materials based on surface area, porosity, and biomass loading.
  • Evaluating reactor performance in both vertical and horizontal orientations.

    • Main Results:

    • The vertical ICR achieved high cell concentrations and dilution rates with continuous operation.
    • Optimal overall productivity for 99% glucose conversion occurred at 15-20% substrate concentration.
    • Packing material with higher surface area and porosity enhanced reactor start-up and cell loading.
    • The vertical reactor demonstrated superior stability (>40 days) compared to the horizontal configuration.
    • Addition of fatty acids to the media limited growth and increased productivity.

    Conclusions:

    • Vertical ICRs with Saccharomyces cerevisiae are stable and highly productive systems.
    • Packing material selection significantly influences reactor performance and start-up time.
    • Vertical orientation promotes mass transfer and liquid distribution, enhancing stability.
    • Process optimization, including fatty acid addition, can further improve ICR efficiency and operational longevity.