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Local and systemic inflammatory responses to lipopolysaccharide in broilers: new insights using a two-window approach
Chelsea E French1, Marites A Sales1, Samuel J Rochell1
1Division of Agriculture, Department of Poultry Science, University of Arkansas, Fayetteville, AR, USA.
Poultry Science
|November 29, 2020
Summary
The growing feather (GF) test in broilers effectively monitors inflammation, showing local tissue responses provide more relevant insights than blood analysis for assessing innate defenses.
Area of Science:
- Veterinary Immunology
- Poultry Science
- Inflammation Research
Background:
- The innate immune system is crucial for broiler health.
- Assessing inflammatory responses in poultry is vital for disease management.
- The growing feather (GF) offers a novel accessible tissue for immune studies.
Purpose of the Study:
- To adapt the GF test for broilers.
- To simultaneously examine local (GF) and systemic (blood) inflammatory responses.
- To compare the efficacy of GF versus blood analysis in assessing inflammation.
Main Methods:
- Broilers were injected with lipopolysaccharide (LPS) in their growing feathers (GF).
- GF pulp and blood samples were collected at 0, 6, and 24 hours post-injection.
- Leukocyte infiltration, gene expression, reactive oxygen species, and superoxide dismutase (SOD) activity were measured.
Main Results:
- LPS injection induced significant changes in GF leukocyte infiltration and gene expression.
- GF analysis revealed elevated reactive oxygen species and SOD activity.
- Blood analysis showed altered leukocyte counts and SOD activity, but GF provided more detailed inflammatory insights.
Conclusions:
- The GF test system is a valuable tool for assessing broiler inflammatory responses.
- Local GF inflammation provides more relevant data than systemic blood markers.
- This method can identify birds with strong innate defenses and evaluate treatments.
