Urea-assisted ensiling process of wilted maize stover for profitable biomethane production

Low contents of water-soluble carbohydrates and/or low indigenous microbial activity in wilted maize stover (WMS) usually hinder the establishment of the ensiling process, thereby resulting in a low biogas production because of high loss of dry matter (DM). To enhance the biological activity and substrate biodegradability, this study applied the synergistic regulation of sucrose (carbon source) and increasing levels of urea (nitrogen source) during the ensiling process of WMS. Compared with the application of only sucrose, a higher organic acid content (lactic acid (85 g/kg-DM) and acetic acid (14 g/kg-DM)) and higher degradation ratios for lignocellulose (hemicellulose (28%), cellulose (22%), and lignin (17%)) were observed with urea applications of 1.7% (DM) and 3.9% (DM), respectively. This was caused by the enhanced activities of the hetero-fermenter (Weissella) and cellulolytic bacteria (Cellulosimicrobium). A simultaneous addition of urea and sucrose during the ensiling of WMS increased the specific methane yield by 11.2%-21.1% in comparison to raw WMS. Moreover, an economic cost estimation revealed that this approach could be an effective storage strategy for the efficient production of methane when employing a 1.7% (DM) urea application.