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Updated: Nov 28, 2025
Assaying Protein Kinase Activity with Radiolabeled ATP
Published on: May 26, 2017
Cationic Polythiophene-based Colorimetric Assay for Probing the Activity of Protein Kinase A
Shanshan Liu1, Sitian Zheng2, Jing Chu2
1Hubei Key Laboratory of Processing and Application of Catalytic Materials, College of Chemistry and Chemical Engineering, Huanggang Normal University, Huanggang, 438000, China. liushanshanlhl@163.com.
Abstract:
In this work, a novel colorimetric assay based on polythiophene derivative (PMNT) was designed for the detection of protein kinase A (PKA). PKA can catalyze the phosphorylation of peptide, leading to the conformation change of PMNT from random-coil to planar, with the disappearance of absorption peaks above 500 nm and a color change from pink to yellow. The fabricated assay exhibits a wide linear range of 0.05 - 20 U/mL with a detection limit of 0.02 U/mL for PKA activity detection. The proposed protocol has promising prospects for use in clinical diagnosis related to PKA activity.
