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Decreased expression of the long non-coding RNA HOXD-AS2 promotes gastric cancer progression by targeting HOXD8 and

Lin Yao1, Peng-Cheng Ye2, Wang Tan3

  • 1Department of Gastrointestinal Surgery, Clinical Medical College and The First Affiliated Hospital of Chengdu Medical College, Chengdu 610000, Sichuan Province, China, The Hepatobiliary Research Institute, North Sichuan Medical College, Nanchong 637000, Sichuan Province, China.

World Journal of Gastrointestinal Oncology
|November 30, 2020
PubMed
Abstract

Insights

Downregulation of the long non-coding RNA HOXD-AS2 promotes gastric cancer (GC) progression by affecting HOXD8 expression and the PI3K/Akt pathway. HOXD-AS2 may serve as a diagnostic biomarker and therapeutic target for GC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) play roles in various cancers, but their specific functions in gastric cancer (GC) remain unclear.
  • Investigating lncRNAs like HOXD-AS2 is crucial for understanding GC development.

Purpose of the Study:

  • To investigate the expression, molecular mechanism, and prognostic significance of HOXD-AS2 in gastric cancer.
  • To determine if HOXD-AS2 can serve as a diagnostic biomarker or therapeutic target for GC.

Main Methods:

  • Real-time quantitative PCR to assess HOXD-AS2 expression in GC tissues and cell lines.
  • In vitro assays (Transwell, wound healing, CCK-8, flow cytometry) to evaluate HOXD-AS2's impact on GC cell proliferation, migration, invasion, cell cycle, and apoptosis.
  • Western blot analysis to explore the regulatory mechanism involving HOXD8 and the PI3K/Akt pathway.

Main Results:

  • Low HOXD-AS2 expression correlated with lymph node metastasis and advanced tumor-node-metastasis stage in GC.
  • Overexpression of HOXD-AS2 inhibited GC cell progression, proliferation, and migration.
  • HOXD-AS2 was found to regulate HOXD8 expression and inhibit the PI3K/Akt signaling pathway.

Conclusions:

  • Downregulation of HOXD-AS2 promotes GC progression by modulating HOXD8 expression and activating the PI3K/Akt pathway.
  • HOXD-AS2 shows potential as a novel diagnostic biomarker and therapeutic target for gastric cancer.
Keywords:
Gastric cancerHOXD-AS2HOXD8Long non-coding RNAPI3K/AktProgression

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