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Optimization of Bacillus cereus Fermentation Process for Selenium Enrichment as Organic Selenium Source
Xujun Chen1, Shuyi Li1, Xin Cong1,2
1National R&D Center for Se-rich Agricultural Products Processing, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China.
Frontiers in Nutrition
|November 30, 2020
Summary
This study enriched the probiotic Bacillus cereus with selenium using sodium selenite. Optimal conditions achieved a 94.3% selenium conversion ratio, yielding organic selenium for potential health applications.
Area of Science:
- Microbiology
- Biotechnology
- Nutritional Science
Background:
- Selenium is a vital micronutrient for human health.
- Organic selenium supplementation in plants and microorganisms is gaining interest.
- Bacillus cereus is a significant probiotic bacterium.
Purpose of the Study:
- To enrich the probiotic Bacillus cereus with selenium.
- To determine optimal fermentation conditions for selenium enrichment.
- To achieve a high organic selenium conversion ratio.
Main Methods:
- Investigated the growth curve of Bacillus cereus.
- Fermented Bacillus cereus with sodium selenite at 150 μg/ml.
- Utilized response surface methodology to optimize fermentation conditions (inoculation, temperature, shaking speed).
Main Results:
- Identified optimal fermentation conditions: 7% inoculation, 33°C, 170 rpm.
- Achieved a maximal selenium conversion ratio of 94.3 ± 0.2%.
- Confirmed inorganic selenium transformation using field emission scanning electron microscopy and energy dispersive spectrometry.
Conclusions:
- Established optimal conditions for selenium enrichment in Bacillus cereus.
- Demonstrated successful transformation of inorganic selenium into an organic form.
- A high selenium conversion ratio strain was developed for selenoprotein extraction.
